MONOVALENT CATION BINDING IN CUBIC INSULIN CRYSTALS
WATER 24 IS PROBABLY A PARTIALLY OCCUPIED SODIUM ION. ANOTHER SODIUM SITE WITH VERY LOW OCCUPANCY OVERLAPS THE MAJOR CONFORMATION OF HIS B10 (SEE REF 3).
Monovalent cation binding to cubic insulin crystals
Structure of the Pig Insulin Dimer in the Cubic Crystal
Water Structure in Cubic Insulin Crystals
Zinc-Free Cubic Pig Insulin: Crystallization and Structure Determination
BY COMPARISON WITH MULTIPLE DATA SETS FROM BOVINE CUBIC INSULIN COLLECTED IN THE PH RANGE 7 TO 11 (REFERENCE 1 AND UNPUBLISHED RESULTS) IT HAS BEEN POSSIBLE TO RELIABLY IDENTIFY ADDITIONAL CONFORMATIONS FOR SEVERAL SIDE CHAINS THAT WERE NOT INCLUDED IN THE PUBLISHED (ACTA CRYSTALLOGR.) REPORT. THESE HAVE BEEN MODELED, THE ORDERED SOLVENT RATIONALIZED AND THE STRUCTURE REFINED FURTHER. THE R-FACTOR AND STEREOCHEMICAL PARAMETERS IN THIS ENTRY ARE FOR THIS NEW MODEL. IN THE ACTA CRYSTALLOGRAPHICA 1991 PUBLICATION (REFERENCE 2 ABOVE) REFERENCE IS MADE TO PROTEIN DATA BANK ENTRY 5INS. THE COORDINATES IN THIS ENTRY SUPERSEDE 5INS.