Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Characterization of a novel format scFv×VHH single-chain biparatopic antibody against metal binding protein MtsA.

Iron ABC transporter substrate-binding lipoprotein MtsA

DKLKVVATNSIIADMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFTKLVKNAQKT
KNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLIAKDPKNKETYEKNLKAYVAKLEKLDKEAKSK
FDAIAENKKLIVTSEGCFKYFSKAYGVPSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSG
IPIYSEIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK

QSLEESGGRLVTPGTPLTLTCTASGFTISRHHMQWVRQAPGKGLEWIGLIDSSGSTYYANWAKGRFTISRTSTTVDLKMT
SLTTEDTATYFCARGGSMDFWGQGTLVTVSSGQPKAPSVGGGGSGGGGSGGGGSAQVLTQTPSPVSAAVGGTVTISCQSS
QSVAGNNFLSWYQQKPGQPPTQLIGATSTLASGVPSRFKGSGSGTQFTLTISDLESDDAATYYCAGGYSGNIFAFGGGTE
LEILRGPHHHHHH

0.05 M Zinc acetate dihydrate, 20% w/v PEG 3350

Japan Society for the Promotion of Science (JSPS)

chain 'B' and (resid 1 through 68 )

chain 'B' and (resid 69 through 178 )

chain 'B' and (resid 179 through 279 )

chain 'D' and (resid 1 through 28 )

chain 'D' and (resid 29 through 42 )

chain 'D' and (resid 43 through 68 )

chain 'D' and (resid 69 through 132 )

chain 'D' and (resid 133 through 279 )

chain 'G' and (resid 1 through 16 )

chain 'G' and (resid 17 through 32 )

chain 'G' and (resid 33 through 58 )

chain 'G' and (resid 59 through 73 )

chain 'G' and (resid 74 through 104 )

chain 'G' and (resid 105 through 153 )

chain 'G' and (resid 154 through 168 )

chain 'G' and (resid 169 through 197 )

chain 'G' and (resid 198 through 242 )

chain 'A' and (resid 1 through 42 )

chain 'A' and (resid 43 through 113 )

chain 'A' and (resid 114 through 279 )

chain 'C' and (resid 1 through 42 )

chain 'C' and (resid 43 through 84 )

chain 'C' and (resid 85 through 185 )

chain 'C' and (resid 186 through 279 )

chain 'E' and (resid 1 through 16 )

chain 'E' and (resid 17 through 50 )

chain 'E' and (resid 51 through 62 )

chain 'E' and (resid 63 through 79 )

chain 'E' and (resid 80 through 153 )

chain 'E' and (resid 154 through 168 )

chain 'E' and (resid 169 through 211 )

chain 'E' and (resid 212 through 242 )

chain 'F' and (resid 1 through 16 )

chain 'F' and (resid 17 through 32 )

chain 'F' and (resid 33 through 50 )

chain 'F' and (resid 51 through 79 )

chain 'F' and (resid 80 through 140 )

chain 'F' and (resid 141 through 153 )

chain 'F' and (resid 154 through 168 )

chain 'F' and (resid 169 through 184 )

chain 'F' and (resid 185 through 197 )

chain 'F' and (resid 198 through 211 )

chain 'F' and (resid 212 through 228 )

chain 'F' and (resid 229 through 242 )

chain 'H' and (resid 1 through 32 )

chain 'H' and (resid 33 through 107 )

chain 'H' and (resid 108 through 145 )

chain 'H' and (resid 146 through 159 )

chain 'H' and (resid 160 through 174 )

chain 'H' and (resid 175 through 197 )

chain 'H' and (resid 198 through 220 )

chain 'H' and (resid 221 through 244 )

Antibody, Metal Transport, METAL TRNASPORT-IMMUNE SYSTEM complex