Rhodothermus marinus alpha-amylase RmGH13_47A CBM48-A-B-C domains in complex with branched pentasaccharide

Structural basis for the recognition of alpha-1,6-branched alpha-glucan by GH13_47 alpha-amylase from Rhodothermus marinus.

GSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGIEDGINYDPNDPTRVTLSLYAPGKSFVYVIGDFTNWEVDPAYFMY
RDAPRPDSVHWWITIEGLTPGQEYAFQYFIDGELRLADLFAHKVLDPWHDPFIPSSTYPNLKPYPTGKTEGIVAVLQPGA
PQYQWQVTDFERPPAHELVIYELLIRDFVARHDYVTLIDTLDYLERLGVNAIELMPVAEFDGNISWGYNPAFHLALDKYY
GPADDLKRFVDECHRRGIAVILDVVYNHATGNSPLVQLYGPTADNPFINIPARHPFNVFYDLNHEHPYIQYWLDRANRYW
LEEFRVDGFRFALSKGFTQKYTDDDVGAWSAYDASRIRLLKRMADAIWAVDSTAYIILEHFADNQEEKELAAYGQDRGRA
GMLLWHNLNRAFSQSVMGYLNDPNFSSDLTTIYYKNRGFPTPNLIAYMESHDEQWLMYRMRAYGARQGAYDVRSLPVALD
RMKLAGAFFFTVPGPKMIWQFGELGYGYGERGEQCLEGTGDSCPSIAPGRIDPKPIRWDYRNDPLRMKLYRTWAELLRLR
REHAVFRSPETQVRMRLQHGVPGRWISLTHPELSVVVVGNFGLEPLVVTPTFPQTGTWYDYFNGDSLVVDDPNTGIELLP
GEFRLYTNRYVGQAE

MES buffer, PEG 20000, PEG 4000, PEG 3350, sodium iodide

Japan Society for the Promotion of Science (JSPS)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

alpha-amylase, (beta/alpha)8 barrel, CARBOHYDRATE, HYDROLASE