Rauvolfia serpentina strictosidine synthase (RsSTR) in complex with a non-reactive tryptamine substitute crystallized in P1211 space group

Proxy-approach in understanding the bisubstrate activity of strictosidine synthases.

SSPILKEILIEAPSYAPNSFTFDSTNKGFYTSVQDGRVIKYEGPNSGFVDFAYASPYWNKAFCENSTDAEKRPLCGRTYD
ISYNLQNNQLYIVDCYYHLSVVGSEGGHATQLATSVDGVPFKWLYAVTVDQRTGIVYFTDVSTLYDDRGVQQIMDTSDKT
GRLIKYDPSTKETTLLLKELHVPGGAEVSADSSFVLVAEFLSHQIVKYWLEGPKKGTAEVLVKIPNPGNIKRNADGHFWV
SSSEELDGNMHGRVDPKGIKFDEFGNILEVIPLPPPFAGEHFEQIQEHDGLLYIGTLFHGSVGILVYDKKGNSFVSSH

0.2 M Sodium chloride, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350

Protein elutes at volume corresponding to molecular weight of polymer but the asymmetric unit of crystal contains eight copies of the protein

Hydrogens have been added in their riding positions

Beta-propeller fold, Bisubstrate enzyme, Pictet-Spengler reaction, Non-substrate anlaog, LYASE

Local NCS retraints between domains: 1 2

Local NCS retraints between domains: 3 4

Local NCS retraints between domains: 5 6

Local NCS retraints between domains: 7 8

Local NCS retraints between domains: 9 10

Local NCS retraints between domains: 11 12

Local NCS retraints between domains: 13 14

Local NCS retraints between domains: 15 16

Local NCS retraints between domains: 17 18

Local NCS retraints between domains: 19 20

Local NCS retraints between domains: 21 22

Local NCS retraints between domains: 23 24

Local NCS retraints between domains: 25 26

Local NCS retraints between domains: 27 28

Local NCS retraints between domains: 29 30

Local NCS retraints between domains: 31 32

Local NCS retraints between domains: 33 34
Local NCS retraints between domains: 35 36
Local NCS retraints between domains: 37 38
Local NCS retraints between domains: 39 40
Local NCS retraints between domains: 41 42
Local NCS retraints between domains: 43 44
Local NCS retraints between domains: 45 46
Local NCS retraints between domains: 47 48
Local NCS retraints between domains: 49 50
Local NCS retraints between domains: 51 52
Local NCS retraints between domains: 53 54
Local NCS retraints between domains: 55 56