Structure of atypical asparaginase from Rhodospirillum rubrum (mutant K19E)

RrA, an enzyme from Rhodospirillum rubrum, is a prototype of a new family of short-chain L-asparaginases.

HHHHHHENLYFQGMAVSPSPLRIFTAGGTIDEDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLDFTEADRE
AIGRAVGQAVEDHILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVGGSDAAFNIGFACAAALMLAPGVYIAMH
GKVFDPAKTRKNRGLGRFEPIDDQE

0.2 uL 17 mg/mL protein in 50 mM HEPES, pH 7.0, 150 mM sodium chloride + 0.2 uL reservoir (Molecular Dimensions Morpheus A2)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Asparaginase, wild type, atypical, enzyme, HYDROLASE

MAVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLDFTEADREAIGRAVGQAVEDH
ILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVGGSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNR
GLGRFEPIDDQE