CRISPR-Cas type III-D effector complex bound to self-target RNA in a post-cleavage state

RNA targeting and cleavage by the type III-Dv CRISPR effector complex.

Particles were monodisperse and homogeneous.

TIGR03984 family CRISPR-associated protein

TIGR03986 family CRISPR-associated RAMP protein

MRGIEITITMQSDWHVGTGMGRGELDSVVQRDGDNLPYIPGKTLTGILRDSCEQVALGLDNGQTRGLWHGWINFIFGDQP
ALAQGAIEPEPRPALIAIGSAHLDPKLKAAFQGKKQLQEAIAFMKPGVAIDAITGTAKKDFLRFEEVVRLGAKLTAEVEL
NLPDNLSETNKKVIAGILASGAKLTERLGGKRRRGNGRCELKFSGYSDQQIQWLKDNYQSVDQPPKYQQNKLQSAGDNPE
QQPPWHIIPLTIKTLSPVVLPARTVGNVVECLDYIPGRYLLGYIHKTLGEYFDVSQAIAAGDLIITNATIKIDGKAGRAT
PFCLFGEKLDGGLGKGKGVYNRFQESEPDGIQLKGERGGYVGQFEQEQRNLPNTGKINSELFTHNTIQDDVQRPTSDVGG
VYSYEAIIAGQTFVAELRLPDSLVKQITSKNKNWQAQLKATIRIGQSKKDQYGKIEVTSGNSADLPKPTGNNKTLSIWFL
SDILLRGDRLNFNATPDDLKKYLENALDIKLKERSDNDLICIALRSQRTESWQVRWGLPRPSLVGWQAGSCLIYDIESGT
VNAEKLQELMITGIGDRCTEGYGQIGFNDPLLSASLGKLTAKPKASNNQSQNSQSNPLPTNHPTQDYARLIEKAAWREAI
QNKALALASSRAKREEILGIKIMGKDSQPTMTQLGGFRSVLKRLHSRNNRDIVTGYLTALEQVSNRKEKWSNTSQGLTKI
RNLVTQENLIWNHLDIDFSPLTITQNGVNQLKSELWAEAVRTLVDAIIRGHKRDLEKAQENESNQQSQGAA

MPAGGRLMKNLYHYHQYEITLESAVDSCKNHLQAAIGLLYSPQKCELVKLDNSGKLVDSYNRLKFNNLGVFEARFFNLNC
ELRWVNESNGNGTAVLLSESDITLTGFEKGLQEFITAIDQQYLLWGEPAKHPPNADGWQRLAEARIGKLDIPLDNPLKPK
DRVFLTSEEYIAEVDDFGNCAVIDERLIKLEVK

MAHHHHHHVGTENLYFQGFLVLIETSGNQHFIFSTNKLRENIGASELTYLATTEILFQGVDRVFQTNYYDQWSDTNSLNF
LADSKLNPAIDDPKNNADIEILLATSGKAIALVKEEGKAKQLIKEVTKQALINAPGLEIGGIYVNCNWQDKLGVAKAVKE
AHKQFEVNRAKRAGANGRFLRLPIAAGCSVSELPASDFDYNADGDKIPVSTVSKVKRETAKSAKKRLRSVDGRLVNDLAQ
LEKSFDELDWLAVVHADGNGLGQILLSLEKYIGEQTNRNYIDKYRRLSLALDNCTINAFKMAIAVFKEDSKKIDLPIVPL
ILGGDDLTVICRGDYALEFTREFLEAFEGQTETHDDIKVIAQKAFGVDRLSACAGISIIKPHFPFSVAYTLAERLIKSAK
EVKQKVTVTNSSPITPFPCSAIDFHILYDSSGIDFDRIREKLRPEDNTELYNRPYVVTAAENLSQAQGYEWSQAHSLQTL
ADRVSYLRSEDGEGKSALPSSQSHALRTALYLEKNEADAQYSLISQRYKILKNFAEDGENKSLFHLENGKYVTRFLDALD
AKDFFANANHKNQGE

MARKVTTRWKITGTLIAETPLHIGGVGGDADTDLALAVNGAGEYYVPGTSLAGALRGWMTQLLNNDESQIKDLWGDHLDA
KRGASFVIVDDAVIHIPNNADVEIREGVGIDRHFGTAANGFKYSRAVIPKGSKFKLPLTFDSQDDGLPNALIQLLCALEA
GDIRLGAAKTRGLGRIKLDDLKLKSFALDKPEGIFSALLDQGKKLDWNQLKANVTYQSPPYLGISITWNPKDPVMVKAEG
DGLAIDILPLVSQVGSDVRFVIPGSSIKGILRTQAERIIRTICQSNGSEKNFLEQLRINLVNELFGSASLSQKQNGKDID
LGKIGALAVNDCFSSLSMTPDQWKAVENATEMTGNLQPALKQATGYPNNISQAYKVLQPAMHVAVDRWTGGAAEGMLYSV
LEPIGVTWEPIQVHLDIARLKNYYHGKEEKLKPAIALLLLVLRDLANKKIPVGYGTNRGMGTITVSQITLNGKALPTELE
PLNKTMTCPNLTDLDEAFRQDLSTAWKEWIADPIDLCQQEAA

MTVGTLGVVGSAKNLKLQLSFINTRQQYVQITLFERNSFKVAEEEFSTELVEIIKTALPTLKNKKVEFEEDGDQIKQIRE
KGQAWVGAAEQIAPYVLPSGNITETPRNVNASNFHNPYNFVPALPRDGITGDLGDCAPAGHSYYHGDKYSGRIAVKLTTV
TPLLIPDASKEEINNNHKTYPVRIGKDGKPYLPPTSIKGMLRSAYEAVTNSRLAVFEDHDSRLAYRMPATMGLQMVPARI
EGDNIVLYPGTSRIGNNGRPANNDPMYAAWLPYYQNRIAYDGSRDYQMAEHGDHVRFWAERYTRGNFCYWRVRQIARHNQ
NLGNRPERGRNYGQHHSTGVIEQFEGFVYKTNKNIGNKHDERVFIIDRESIEIPLSRDLRRKWRELITSYQEIHKKEVDR
GDTGPSAVNGAVWSRQIIADESERNLSDGTLCYAHVKKEDGQYKILNLYPVMITRGLYEIAPVDLLDETLKPATDKKQLS
PADRVFGWVNQRGNGCYKGQLRIHSVTCQHDDAIDDFGNQNFSVPLAILGQPKPEQARFYCADDRKGIPLEDGYDRDDGY
SDSEQGLRGRKVYPHHKGLPNGYWSNPTEDRSQQAIQGHYQEYRRPKKDGLEQRDDQNRSVKGWVKPLTEFTFEIDVTNL
SEVELGALLWLLTLPDLHFHRLGGGKPLGFGSVRLDIDPDKTDLRNGAGWRDYYGSLLETSQPDFTTLISQWINAFQTAV
KEEYGSSSFDQVTFIKASGQSLQGFHDNASIHYPRSTPEPKPDGEAFKWFVANEKGRRLALPALEKSQSFPIKPS

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

CRISPR, CRISPR-Cas, type III, complex, RNA, crRNA, RNA BINDING PROTEIN-RNA complex

FLVLIETSGNQHFIFSTNKLRENIGASELTYLATTEILFQGVDRVFQTNYYDQWSDTNSLNFLADSKLNPAIDDPKNNAD
IEILLATSGKAIALVKEEGKAKQLIKEVTKQALINAPGLEIGGIYVNCNWQDKLGVAKAVKEAHKQFEVNRAKRAGANGR
FLRLPIAAGCSVSELPASDFDYNADGDKIPVSTVSKVKRETAKSAKKRLRSVDGRLVNDLAQLEKSFDELDWLAVVHADG
NGLGQILLSLEKYIGEQTNRNYIDKYRRLSLALDNCTINAFKMAIAVFKEDSKKIDLPIVPLILGGDDLTVICRGDYALE
FTREFLEAFEGQTETHDDIKVIAQKAFGVDRLSACAGISIIKPHFPFSVAYTLAERLIKSAKEVKQKVTVTNSSPITPFP
CSAIDFHILYDSSGIDFDRIREKLRPEDNTELYNRPYVVTAAENLSQAQGYEWSQAHSLQTLADRVSYLRSEDGEGKSAL
PSSQSHALRTALYLEKNEADAQYSLISQRYKILKNFAEDGENKSLFHLENGKYVTRFLDALDAKDFFANANHKNQGE