NMR solution structure of the CysD2 domain of MUC2

The structure of the second CysD domain of MUC2 and role in mucin organization by transglutaminase-based cross-linking.

DAAQPARRAVRSSSELTSEQKLISEEDLSSPCVPLCNWTGWLDSGKPNFHKPGGDTELIGDVCGPGWAANISCRATMYPD
VPIGQLGQTVVCDVSVGLICKNEDQKPGGVIPMAFCLNYEINVQCCECVTQPTTMTTTTTEN

6 CALCIUM BOND DISTANCES, 14 HYDROGEN BONDS, 5 DISULFIDE BONDS, YASARA

U13C15N labeling
10 mM phosphate
100 mM NaCl
pH 7.0

0.6 mM 15N Cysteine, 15N Valine cysD2, 90% H2O/10% D2O

colon, transglutaminase, isopeptide bond, structural protein

PCVPLCNWTGWLDSGKPNFHKPGGDTELIGDVCGPGWAANISCRATMYPDVPIGQLGQTVVCDVSVGLICKNEDQKPGGV
IPMAFCLNYEINVQCCECVTQPTTMTTTTTEN