Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8b

Discovery and characterization of potent spiro-isoxazole-based cereblon ligands with a novel binding mode.

AMPLDAGGQNSTQMVLAPGASIFRCRQCGQTISRRDWLLPMGGDHEHVVFNPAGMIFRVWCFSLAQGLRLIGAPSGEFSW
FKGYDWTIALCGQCGSHLGWHYEGGSQPQTFFGLIKDRLAEGPAD

0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

CEREBLON, Ubiquitination, E3, MOLECULAR GLUE, spiro-isoxazole, SIGNALING PROTEIN

MPLDAGGQNSTQMVLAPGASIFRCRQCGQTISRRDWLLPMGGDHEHVVFNPAGMIFRVWCFSLAQGLRLIGAPSGEFSWF
KGYDWTIALCGQCGSHLGWHYEGGSQPQTFFGLIKDRLAEGPAD