Cryo-EM structure of Pyrococcus furiosus apo form RNA polymerase contracted clamp conformation

Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.

Pyrococcus furiosus apo form of RNA polymerase contracted clamp conformation

DNA-directed RNA polymerase subunit Rpo1N

DNA-directed RNA polymerase subunit beta

DNA-directed RNA polymerase subunit Rpo1C

DNA-directed RNA polymerase subunit Rpo3

DNA-directed RNA polymerase subunit Rpo7

DNA-directed RNA polymerase subunit Rpo4

DNA-directed RNA polymerase subunit Rpo5

DNA-directed RNA polymerase subunit Rpo6

DNA-directed RNA polymerase subunit Rpo11

DNA-directed RNA polymerase subunit Rpo10

DNA-directed RNA polymerase subunit Rpo12

MQSMKKVIGSIEFGILSPQEIRKMSAVEVTVPDTYDDDGYPIEGGVMDKRMGVIDPGLRCETCGGRAGECPGHFGHIELA
RPVIHVGFAKTIYRILESTCRECGRIKLTDEEIEEYMKKIELARNRRSEFNEIIKEIHKKAKERMVCPHCGAPQYPIKFE
KPTIYWEIRKDEQGNEYRHRLMPTEVRDWLEKIPDKDLPLLGLDPEKSRPEWMVLTVLPVPPVTARPSITLETGIRAEDD
LTHKLVDIIRINNRLKQNIEAGAPQLIIEDLWDLLQYHVTTYINNEAPGVPPAKHKSGRPLKTLAQRLKGKEGRFRGNLS
GKRVNFSARTVISPDPMISINEVGVPVEVAMELTVPEKVTEFNIERLRKMVLNGPDKYPGANYVIDPEGRRRRIMDSNKE
TLANQLDIGWTVERHLMDGDIVLFNRQPSLHRMSIMAHRVRVMPYRTFRLNLAVCPPYNADFDGDEMNLHVPQTEEAQAE
ARILMEVQNHIISPRYGGPIIGGIQDHISGGYLLTREGAYFTRDEVEQMLMFAGVDITELPEPDKYDENGNPLWSGKTIF
SLLLPEDLTVWYRNKLCDEPERCEALEKLIEEKLMPDPEEVRKLAYDGFVYIQNGKLLSGAIDKKAYGREDGIILDLIVR
EYGVERARQFLDQVTKLTIWVITHKGFTTGIDDEDLPEEARDRIREIIREAEERVNKLIEAYKRGELEPLPGKSLEDTLE
SLIMAVLAEARDNAGAVAEKYLGMDNHTVIMAKTGARGKILNITQMAALLGQQSIRGKRLYRGFRGRVLSHFKPGDLGAR
AKGFVVNSYKSGLTPQEYFFHAMGGREGLVDTAVRTAQSGYMQRRLINALQDLKVEYDGTVRSPEGIIVQFKYGEDGVDP
MKSWRGKTVDVDRIIVRTLLKMRGNNKAKV

MRGPTVVDVTPDDLWIVMESYWQEKGLVRQHLDSYNAFIERGLQEVVNEFGGIKPDIPDFEVKFGKIRVGEPEFQEPHGQ
RKPLYPMDARIRNLTYAAPLYLEMIPVIKGVEQDPVEVRIGELPIMLKSKVCRLYGLSDEELIKLGEDPKDPGGYFIING
SERVIVSIEDIAPNRTLVEKDERQEKYIAKVFSYRHGYRALVAVERRKDGILYVDIPNVPKPVKFVYVMRALGLERDKDI
VEAVGNDPEVQQIMFDNLEDASDITTQQEALEFIGKLVAPGQAREYRLKRAEYVIDNNLLPHMGVSPEDRIKKAYYLGMM
ALKVIELSLGRRPEDDKDHYANKRLRLAGDLLKDLFRVAFSQLVKDIQYQMTKTYQRKGDKYTFGNIHRFIRNSIRPDVL
TERIEHALATGAWPGGRTGVSQLLDRTNYMSTLSHLRRVTSPLDREQPHFEARDLHGTHWGRICPTETPEGPNCGLVKNL
ALMAQITTAVPEEEIREYLMSLGIVPIEVRRPDPTLWRVYLNGVLIGTIEDGKALVQRIRQDRRSGKISDVINVAYYEDV
KEVYVNSDDGRVRRPLIIVENGVPKLTKEHVQAVKEGRLKWSDLIKMGIIEYLDAEEEENAYVAMWPWEVTEEHTHLELM
PAAILGLPASLVPYPEHNAAPRNTYGAGMAKQSLGLGWANFRIRVDTRGHLLHYPQIPLVNSRIMKAVGYEDRPAGQNFV
VAVLAYQGYNMEDAIIINKASIERGLARSTFFRTYEAEEKKYLGGQTDKFEIPDPTVRGYRGEKYYRNLDEDGLIFPESK
VEGKDVLVGRTSPPRFLEERGLGTVSLQERRETSVAVRPSEKGVVDKVIITETGDGTKLVKVTVRDLRIPELGDKFASRH
GQKGVIGLIVPQEDMPWTESGIVPDLIVNPHGIPSRMTVGQLIEAIGGKVASLKGRRVDGTAFIGEPEEKLRKELEELGF
KHTGREIMYDGITGKRLEADIFIGVIYYQRLHHMVADKIHARSRGPVQVLTKQPTEGRAREGGLRFGEMERDVLVGHGAA
MLLIERLLEESDKTEVWVCENCGHIALEDKRRRRVYCPVCGEEERISKVEMSYAFKLLLDELKAMVIRPKLNLSERV

MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALVEPGEPVGTVAAQSIGEPSTQ
MTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTVYLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFE
FIVEIDPERLERSGLTMEKVVKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGLEVDIRHIMLVADMMTLDGIV
RPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNLNGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV

MAGIEVQILEKKEDSIKFVLKGVHVSFANALRRTILGEVPTFAVDEVEFYENDSALFDEIIAHRLAMIPLTTPVDRFELD
ALELDDYTVTLSLEAEGPGIVYSGDLKSDDPDVKPVNPNIPIVKLAEGQRLVFNAYAKLGRGKDHAKWQPGFVYYKYYTI
VHISKSIPEWKELKKLAKKRGLPVEETEEEVLVTTIKPFYIPKDFEEYEGKEIWEEIVPNTYIFTVETNGELPVEEIVSI
ALKILMRKADRFISELQKLTSWSHPQFEKHHHHHH

MYKIVTVKDVVRIPPTMFTMDPKEAAKIILRETYEGTYDKDEGVILSILEVKDIKDGIIIPGDGATYHEVVFDVLVWEPK
IHEVVEGYVADVMPFGAFIRIGPIDGLVHISQLMDDYVVYDERNKQFVGKEKKYLLKIGDLVRARIINISAKSKVIRENR
IGLTMRQPGLGKFEWIEKEKKKEKEEGKK

MIGRGKLGEKYITIAEAKELLLKRREEEVKAGIEEPLYYEARLALEHAERFAKLPADKAKEAVEELMNAFEWMSDRIACK
IVDIMPEDSMDLRVIFAKEEYQPTQEEMKQILDILDKYRE

MAGKKEFSIFDHVLVPEHRVLSEEEKKALLEKYKITLAQLPQIKASDPAVKALGAKPGDVIEIKRKSPTAGVYYYYRVVV
ED

MKIEVIKKEENLLEFYLEGEDHTFANLLVETLRENPHVKFTAYTIEHPITMARKPRFRVVTDGEITPEEALEEAAKKIFE
RAKEVLEAWEKAVKS

RNA, Polymerase, Elongation, Complex, Pyrococcus furiosus, Archaea, Transcription, DNA

MAGIEVQILEKKEDSIKFVLKGVHVSFANALRRTILGEVPTFAVDEVEFYENDSALFDEIIAHRLAMIPLTTPVDRFELD
ALELDDYTVTLSLEAEGPGIVYSGDLKSDDPDVKPVNPNIPIVKLAEGQRLVFNAYAKLGRGKDHAKWQPGFVYYKYYTI
VHISKSIPEWKELKKLAKKRGLPVEETEEEVLVTTIKPFYIPKDFEEYEGKEIWEEIVPNTYIFTVETNGELPVEEIVSI
ALKILMRKADRFISELQKLTS