Crystal structure of the human DNPH1 glycosyl-enzyme intermediate

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Mechanism of substrate hydrolysis by the human nucleotide pool sanitiser DNPH1.

2'-deoxynucleoside 5'-phosphate N-hydrolase 1

GSMGRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTQHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVT
QPSLGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADP

0.1 M Bis-Tris propane pH 6.5,
16% w/v PEG 3350

Medical Research Council (MRC, United Kingdom)

Crystal structure of human DNPH1 bound to hmdUMP

DNPH1, N-glycosidase, hmdUMP, dRP, HYDROLASE

GRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPS
LGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADP