Human IgD Fab in complex with an orthosteric inhibitor of Phl p 7

Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor.

(PCA)SALTQPPSVSGAPGQRVSISCTGGSSNFGAGYDVHWYQQLPATAPKLLIYGNNNRPSGVPDRFSGSKSGTSASLA
ITGLQAEDEGDYFCQSFDTSLSGWIFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

QSALTQPPSVSGAPGQRVSISCTGGSSNFGAGYDVHWYQQLPATAPKLLIYGNNNRPSGVPDRFSGSKSGTSASLAITGL
QAEDEGDYFCQSFDTSLSGWIFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPV
KAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

(PCA)VQLVQSGAEVRNPGASVKVSCKASGYTFTSYAIHWVRQAPGHRLEWVGRINTDNGNTKYSQKFHGRVALSRDTSA
STTYMDLSSLNSEDTAVYYCARAFYYSSGVMFDSWGQGALVTVSSAPTKAPDVFPIISGCRHPKDNSPVVLACLITGYHP
TSVTVTWYMGTQSQPQRTFPEIQRRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKA

QVQLVQSGAEVRNPGASVKVSCKASGYTFTSYAIHWVRQAPGHRLEWVGRINTDNGNTKYSQKFHGRVALSRDTSASTTY
MDLSSLNSEDTAVYYCARAFYYSSGVMFDSWGQGALVTVSSAPTKAPDVFPIISGCRHPKDNSPVVLACLITGYHPTSVT
VTWYMGTQSQPQRTFPEIQRRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKA

MQVQLQESGGGLVQAGGSLRLSCAASGFTFDDYAIGWFRQAPGKEREGVSGIRRSDGSTHYADSVKGRFTISTDNAKNTV
YLQMNNLKPEDTAVYYCAAAGTPSYYYTEPLSLGTYDYWGQGTQVTVSSAAAENLYFQGGSHHHHHH

15% PEG 8000, 0.1M sodium citrate and 0.05M ammonium sulfate

Medical Research Council (MRC, United Kingdom)

Fab, Phl p 7, nanobody, IgD, IMMUNE SYSTEM