Local refinement of cubic assembly from truncated PVY coat protein with K176C mutation

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y.

Final cryoEM map was obtained by local refinement from C4 symmetry expanded particles.

1.8 mM KH2PO4, 10.1 mM Na2HPO4, 140 mM NaCl, 2.7 mM KCl, pH 7.4

truncated coat protein (dN49C40) with C-terminal His tag and K176C mutation

GITSKMRMPKSKGATVLNLEHLLEYAPQQIDISNTRATQSQFDTWYEAVQLAYDIGETEMPTVMNGLMVWCIENGTSPNI
NGVWVMMDGDEQVEYPLKPIVENAKPTLRQIMAHFSDVAEAYIEMRNCKEPYMPRYGLVRNLRDGSLARYAFDFYEVTSR
TPVRAREAHIQMKAAALKSENLYFQGLEHHHHHH

Ministry of Education, Youth and Sports of the Czech Republic

EMD-17062 contains global refinement cryoEM map with rigid-body docked and merged atomic model

cubes, trCP, K176C, Potyvirus, PVY, VIRUS LIKE PARTICLE

ITSKMRMPKSKGATVLNLEHLLEYAPQQIDISNTRATQSQFDTWYEAVQLAYDIGETEMPTVMNGLMVWCIENGTSPNIN
GVWVMMDGDEQVEYPLKPIVENAKPTLRQIMAHFSDVAEAYIEMRNKKEPYMPRYGLVRNLRDGSLARYAFDFYEVTSRT
PVRAREAHIQMKAAALKSAQSRLFGLD