Virus-like Particle based on PVY coat protein with stacked-ring architecture

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y.

cryoSPARC Helical refinement with initial helical parameters from EMD-0298

1.8 mM KH2PO4, 10.1 mM Na2HPO4, 140 mM NaCl, 2.7 mM KCl, pH 7.4

The sample contains 3 forms of filaments.
Virus-like particle from PVY coat protein

Due to flexibility, structural model could only be built from H42 to A222.

GNDTIDAGGSTKKDAKQEQGSIQPNLNKEKEKDVNVGTSGTHTVPRIKAITSKMRMPKSKGATVLNLEHLLEYAPQQIDI
SNTRATQSQFDTWYEAVQLAYDIGETEMPTVMNGLMVWCIENGTSPNINGVWVMMDGDEQVEYPLKPIVENAKPTLRQIM
AHFSDVAEAYIEMRNKKEPYMPRYGLVRNLRDGSLARYAFDFYEVTSRTPVRAREAHIQMKAAALKSAQSRLFGLDGGIS
TQEENTERHTTEDVSPSMHTLLGVKNM

EMD-17047 contains same protein in VLPh form.

8OPB contains the model of same protein in VLPh form.

stacked-ring, VLP, Potyvirus, PVY, VIRUS LIKE PARTICLE