The crystal structure of syn-copalyl diphosphate synthase from Oryza sativa

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural and functional investigations of syn-copalyl diphosphate synthase from Oryza sativa.

Syn-copalyl diphosphate synthase, chloroplastic

MPVFTASFQCVTLFGQPASAADAQPLLQGQRPFLHLHARRRRPCGPMLISKSPPYPASEETREWEADGQHEHTDELRETT
TTMIDGIRTALRSIGEGEISISAYDTSLVALLKRLDGGDGPQFPSTIDWIVQNQLPDGSWGDASFFMMGDRIMSTLACVV
ALKSWNIHTDKCERGLLFIQENMWRLAHEEEDWMLVGFEIALPSLLDMAKDLDLDIPYDEPALKAIYAERERKLAKIPRD
VLHSMPTTLLHSLEGMVDLDWEKLLKLRCLDGSFHCSPASTATAFQQTGDQKCFEYLDGIVKKFNGGVPCIYPLDVYERL
WAVDRLTRLGISRHFTSEIEDCLDYIFRNWTPDGLAHTKNCPVKDIDDTAMGFRLLRLYGYQVDPCVLKKFEKDGKFFCL
HGESNPSSVTPMYNTYRASQLKFPGDDGVLGRAEVFCRSFLQDRRGSNRMKDKWAIAKDIPGEVEYAMDYPWKASLPRIE
TRLYLDQYGGSGDVWIGKVLHRMTLFCNDLYLKAAKADFSNFQKECRVELNGLRRWYLRSNLEKFGGTDPQTTLMTSYFL
ASANIFEANRAAERLGWARVALLADAVSSHFRRIGGPKNSTSNLEELISLVPFDDAYSGSLREAWKQWLMAWTAKESSQE
SIEGDTAILLVRAIEIFGGRHVLTGQRPDLWEYSQLEQLTSSICCKLSRRVLAQENGESTEKVEEIDQQVDLEMQELTRR
VLQGCSAINRLTRETFLHVVKSFCYVAYCSPETIDSHIDKVIFQDVIEFHHHHHH

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

2% v/v 1,4-dioxane, 0.1 M Tris pH 8.0, 15% w/v polyethylene glycol 3,350

syn-copalyl diphosphate synthase, labdane-related diterpenoids, apo-state crystal structure, plant defense, PLANT PROTEIN, ISOMERASE
(chain 'A' and (resid 81 through 113 or resid 124 through 139 or resid 142 through 184 or resid 195 through 209 or resid 221 through 226 or resid 237 through 401 or resid 407 through 452 or resid 457 through 538 or resid 540 through 544 or resid 551 through 694 or resid 696 through 767))
(chain 'B' and (resid 81 through 113 or resid 124 through 139 or resid 142 through 209 or resid 221 through 226 or resid 237 through 401 or resid 407 through 538 or resid 540 through 544 or resid 551 through 767))
(chain 'C' and (resid 81 through 184 or resid 195 through 226 or resid 237 through 452 or resid 457 through 538 or resid 540 through 544 or resid 551 through 694 or resid 696 through 767))
(chain 'D' and (resid 81 through 113 or resid 124 through 139 or resid 142 through 184 or resid 195 through 209 or resid 221 through 226 or resid 237 through 401 or resid 407 through 452 or resid 457 through 538 or resid 540 through 544 or resid 551 through 694 or resid 696 through 767))
(chain 'E' and (resid 81 through 113 or resid 124 through 139 or resid 142 through 184 or resid 195 through 209 or resid 221 through 226 or resid 237 through 401 or resid 407 through 452 or resid 457 through 538 or resid 540 through 544 or resid 551 through 694 or resid 696 through 767))
(chain 'F' and (resid 81 through 113 or resid 124 through 139 or resid 142 through 184 or resid 195 through 209 or resid 221 through 226 or resid 237 through 401 or resid 407 through 452 or resid 457 through 538 or resid 540 through 694 or resid 696 through 767))

MPVFTASFQCVTLFGQPASAADAQPLLQGQRPFLHLHARRRRPCGPMLISKSPPYPASEETREWEADGQHEHTDELRETT
TTMIDGIRTALRSIGEGEISISAYDTSLVALLKRLDGGDGPQFPSTIDWIVQNQLPDGSWGDASFFMMGDRIMSTLACVV
ALKSWNIHTDKCERGLLFIQENMWRLAHEEEDWMLVGFEIALPSLLDMAKDLDLDIPYDEPALKAIYAERERKLAKIPRD
VLHSMPTTLLHSLEGMVDLDWEKLLKLRCLDGSFHCSPASTATAFQQTGDQKCFEYLDGIVKKFNGGVPCIYPLDVYERL
WAVDRLTRLGISRHFTSEIEDCLDYIFRNWTPDGLAHTKNCPVKDIDDTAMGFRLLRLYGYQVDPCVLKKFEKDGKFFCL
HGESNPSSVTPMYNTYRASQLKFPGDDGVLGRAEVFCRSFLQDRRGSNRMKDKWAIAKDIPGEVEYAMDYPWKASLPRIE
TRLYLDQYGGSGDVWIGKVLHRMTLFCNDLYLKAAKADFSNFQKECRVELNGLRRWYLRSNLEKFGGTDPQTTLMTSYFL
ASANIFEANRAAERLGWARVALLADAVSSHFRRIGGPKNSTSNLEELISLVPFDDAYSGSLREAWKQWLMAWTAKESSQE
SIEGDTAILLVRAIEIFGGRHVLTGQRPDLWEYSQLEQLTSSICCKLSRRVLAQENGESTEKVEEIDQQVDLEMQELTRR
VLQGCSAINRLTRETFLHVVKSFCYVAYCSPETIDSHIDKVIFQDVI