CrtSPARTA hetero-dimer bound with guide-target, state 1

Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system.

RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3')

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMKELIYIEEPSILFAHGQKCTDPRDGLALFGPLNQIYGIKSGVVGT
QKGLQIFKSYLDKIQKPIYNHNNITRPMFPGFEAVFGCKWESQNIVFKEITDEEIRRYLFNASTHKRTYDLVTLFNDKII
TANKNDEERVDVWFVIVPEEIYKYCRPNSVLPNELVQTKSLISKSKAKSFRYTPTLFEEFNKKLKEVEKEAKTYNYDAQF
HDQLKARLLEHTIPTQILRESTLAWRDFKNTFGAPIRDFSKIEGHLAWTISTAAYYKAGGKPWKLGDIRPGVCYLGLVYK
KIEKSKNPQNACCAAQMFLDNGDGTVFKGEVGPWYNPEKGEYHLKPKEAKALLTQALESYKEQNKSYPKEVFIHARTRFN
DEEWNAFNEVTPKNTNLVGVTITKSKPLKLYKTEGAFPIMRGNAYIVDEKKAFLWTLGFVPKLQSTLSMEVPNPIFIEIN
KGEAEIQQVLKDILALTKLNYNACIYADGEPVTLRFANKIGEILTASTEIKTPPLAFKYYI

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMRNKIFISHATPEDDDFTRWLSLKLIGLGYEVWCDILFLDKGVDFW
STIEKEIRENTCKFLIVSSTAGNKREGVLKELAVATKVKKHLQDDMFIIPLAIDENLSYDDINIEIVRLNAIDFKKSWAK
GLQDLLDAFEKQNVPKKPPDHSKSNLLYQQIFLHDKQAIEKEETYDSNWFPIISFPNELRFHRYDWRLPKQFDVRTLAFP
AIRYKEYLCTFAWEYDFIHQLPKTETYNGQESIRISTSDILSGRYDTDFIRNYECQRLIVQLINKAFELRMKDKNVREYQ
MSKTFAYWIEKGKLEKDKFEKIKLVGKQKNKYWHFGISAAGKLYPSPVLMVSSHIIFTMDGINLIKSKSIQHSSRRKQGK
NWWNDKWREKLLAFIRFLSDDQNAIYLNVGSEEKILISNKPLKFFGKMSYVTPSEVTLEEESVLADINNFEEDTEDLDEL
EDIE

(DC)(DA)(DA)(DC)(DT)(DA)(DA)(DT)(DA)(DG)(DA)(DT)(DT)(DA)(DG)(DA)(DG)(DC)(DC)(DG)
(DT)(DC)(DA)(DA)(DT)

Ministry of Science and Technology (MoST, China)

National Natural Science Foundation of China (NSFC)

SPARTA, Ago, Tir, DNA BINDING PROTEIN/DNA/RNA, DNA BINDING PROTEIN-DNA-RNA complex

MKELIYIEEPSILFAHGQKCTDPRDGLALFGPLNQIYGIKSGVVGTQKGLQIFKSYLDKIQKPIYNHNNITRPMFPGFEA
VFGCKWESQNIVFKEITDEEIRRYLFNASTHKRTYDLVTLFNDKIITANKNDEERVDVWFVIVPEEIYKYCRPNSVLPNE
LVQTKSLISKSKAKSFRYTPTLFEEFNKKLKEVEKEAKTYNYDAQFHDQLKARLLEHTIPTQILRESTLAWRDFKNTFGA
PIRDFSKIEGHLAWTISTAAYYKAGGKPWKLGDIRPGVCYLGLVYKKIEKSKNPQNACCAAQMFLDNGDGTVFKGEVGPW
YNPEKGEYHLKPKEAKALLTQALESYKEQNKSYPKEVFIHARTRFNDEEWNAFNEVTPKNTNLVGVTITKSKPLKLYKTE
GAFPIMRGNAYIVDEKKAFLWTLGFVPKLQSTLSMEVPNPIFIEINKGEAEIQQVLKDILALTKLNYNACIYADGEPVTL
RFANKIGEILTASTEIKTPPLAFKYYI

MRNKIFISHATPEDDDFTRWLSLKLIGLGYEVWCDILFLDKGVDFWSTIEKEIRENTCKFLIVSSTAGNKREGVLKELAV
ATKVKKHLQDDMFIIPLAIDENLSYDDINIEIVRLNAIDFKKSWAKGLQDLLDAFEKQNVPKKPPDHSKSNLLYQQIFLH
DKQAIEKEETYDSNWFPIISFPNELRFHRYDWRLPKQFDVRTLAFPAIRYKEYLCTFAWEYDFIHQLPKTETYNGQESIR
ISTSDILSGRYDTDFIRNYECQRLIVQLINKAFELRMKDKNVREYQMSKTFAYWIEKGKLEKDKFEKIKLVGKQKNKYWH
FGISAAGKLYPSPVLMVSSHIIFTMDGINLIKSKSIQHSSRRKQGKNWWNDKWREKLLAFIRFLSDDQNAIYLNVGSEEK
ILISNKPLKFFGKMSYVTPSEVTLEEESVLADINNFEEDTEDLDELEDIE