Selenomethionine mutant (L601Sem/L654Sem) of SAND domain of protein SP140 with DNA

Molecular basis for Speckled protein SP140 bivalent recognition of histone H3 and DNA

DNA (5'-D(*GP*GP*GP*CP*GP*GP*CP*CP*GP*CP*CP*CP*T)-3')

Lymphoid-restricted homolog of Sp100,LYSp100,Nuclear autoantigen Sp-140,Speckled 140 kDa

GPLGSDETVDFKAPLLPVTCGGVKGI(MSE)HKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWP
LRW(MSE)(MSE)ENGFLPDPPRIRYRKKKR

GPLGSDETVDFKAPLLPVTCGGVKGIMHKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWM
MENGFLPDPPRIRYRKKKR

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

0.12 M DL-Malic acid pH 7.0, 14% w/v PEG 3350 0.025% v/v Dichloromethane

National Natural Science Foundation of China (NSFC)

ITC results indicate that the binding ratio of SAND domain and dsDNA is 2:1

Nuclear body protein SP140 SAND domain DNA, DNA BINDING PROTEIN/DNA, DNA BINDING PROTEIN-DNA complex

DETVDFKAPLLPVTCGGVKGILHKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWLMENGF
LPDPPRIRYRKKKR