Crystal structure of Flavihumibacter petaseus GH31 alpha-galactosidase mutant D304A in complex with alpha-1,4-galactobiose

beta-D-galactose; D-galactose; galactose

alpha-D-galactose; D-galactose; galactose; ALPHA D-GALACTOSE

Structure-function analysis of bacterial GH31 alpha-galactosidases specific for alpha-(1→4)-galactobiose.

MGSSHHHHHHSSGLVPRGSHMASQQARQQSLHIPLLKGECWWGAAVNRAHDMPLQPGAFIQLNGDVSGNQAVPLLLSSAG
RYVWSDQPFSVKREGDILSISFTGTGALYTASGGSLKDAWGEAAARFFPASGRLPDTSLFTAPQYNTWIELIYNQNQEDI
LRYARDIVANGFPPGVLMIDDNWFPYYGNFSFRKDRFPDAAGMISTLHGMGFKVMLWVCPFLSPDTEAFREALAKRIVLF
DSKGSDTLQWQHAVDPAIVHWWNGYSAVLDGSNPDAVTWMREKLDGLQQQYGIDGFKFAAGDAEFYLGNILSREKIGANE
QCERWGRIGLLYPMNEYRAMWKNGGQPLVERLRDKYHTWEDVRKLIPHASLAGLLGYSFVCPDMIGGGDFSSFLNNNKLD
QELIVRSAQCHALMPMMQFSVAPWRVLDSSQLQAVKNAVALRRQMLPEIMKYTREAAVTGMPVLRSMEFVFPHQGFERVE
DQFMLGDNYLVAPVLEKGSVRKIKLPKGRWQEIQSGKVYRGGETIELKVTLNTIPCFKRTT

1 M sodium chloride, 50 mM MES-NaOH, pH 6.0

Japan Society for the Promotion of Science (JSPS)

Hydrogens have been added in their riding positions

Glycoside hydrolase, (beta/alpha)8 barrel, Globo sphingolipid, glycolipid, Carbohydrate, HYDROLASE

QQARQQSLHIPLLKGECWWGAAVNRAHDMPLQPGAFIQLNGDVSGNQAVPLLLSSAGRYVWSDQPFSVKREGDILSISFT
GTGALYTASGGSLKDAWGEAAARFFPASGRLPDTSLFTAPQYNTWIELIYNQNQEDILRYARDIVANGFPPGVLMIDDNW
FPYYGNFSFRKDRFPDAAGMISTLHGMGFKVMLWVCPFLSPDTEAFREALAKRIVLFDSKGSDTLQWQHAVDPAIVHWWN
GYSAVLDGSNPDAVTWMREKLDGLQQQYGIDGFKFDAGDAEFYLGNILSREKIGANEQCERWGRIGLLYPMNEYRAMWKN
GGQPLVERLRDKYHTWEDVRKLIPHASLAGLLGYSFVCPDMIGGGDFSSFLNNNKLDQELIVRSAQCHALMPMMQFSVAP
WRVLDSSQLQAVKNAVALRRQMLPEIMKYTREAAVTGMPVLRSMEFVFPHQGFERVEDQFMLGDNYLVAPVLEKGSVRKI
KLPKGRWQEIQSGKVYRGGETIELKVTLNTIPCFKRTT