Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis in complex with glycerol

Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis ( Bi Bga42A) revealed by crystallographic and mutational analyses.

Bifidobacterium longum subsp. infantis uses two different beta-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides.

Numerical link type Si(111) double crystal

MEHRAFKWPQPLAGNKPRIWYGGDYNPDQWPEEVWDEDVALMQQAGVNLVSVAIFSWAKLEPEEGVYDFDWLDRVIDKLG
KAGIAVDLASGTASPPMWMTQAHPEILWVDYRGDVCQPGARQHWRATSPVFLDYALNLCRKMAEHYKDNPYVVSWHVSNE
YGCHNRFDYSEDAERAFQKWCEKKYGTIDAVNDAWGTAFWAQRMNNFSEIIPPRFIGDGNFMNPGKLLDWKRFSSDALLD
FYKAERDALLEIAPKPQTTNFMVSAGCTVLDYDKWGHDVDFVSNDHYFSPGEAHFDEMAYAACLTDGIARKNPWFLMEHS
TSAVNWRPTNYRLEPGELVRDSLAHLAMGADAICYFQWRQSKAGAEKWHSAMVPHAGPDSQIFRDVCELGADLNKLADEG
LLSTKLVKSKVAIVFDYESQWATEHTATPTQEVRHWTEPLDWFRALADNGLTADVVPVRGPWDEYEAVVLPSLAILSEQT
TRRVREYVANGGKLFVTYYTGLVDDRDHVWLGGYPGSIRDVVGVRVEEFAPMGTDAPGTMDHLDLDNGTVAHDFADVITS
VADTAHVVASFKADKWTGFDGAPAITVNDFGDGKAAYVGARLGREGLAKSLPALLEELGIETSAEDDRGEVLRVERADET
GENHFVFLFNRTHDVAVVDVEGEPLVASLAQVNESEHTAAIQPNGVLVVKLAAALEHHHHHH

Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

0.1 M KSCN, 30% PEG MME 2000, 25% glycerol

Japan Society for the Promotion of Science (JSPS)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Glycoside Hydrolase family 42, HYDROLASE

MEHRAFKWPQPLAGNKPRIWYGGDYNPDQWPEEVWDEDVALMQQAGVNLVSVAIFSWAKLEPEEGVYDFDWLDRVIDKLG
KAGIAVDLASGTASPPMWMTQAHPEILWVDYRGDVCQPGARQHWRATSPVFLDYALNLCRKMAEHYKDNPYVVSWHVSNE
YGCHNRFDYSEDAERAFQKWCEKKYGTIDAVNDAWGTAFWAQRMNNFSEIIPPRFIGDGNFMNPGKLLDWKRFSSDALLD
FYKAERDALLEIAPKPQTTNFMVSAGCTVLDYDKWGHDVDFVSNDHYFSPGEAHFDEMAYAACLTDGIARKNPWFLMEHS
TSAVNWRPTNYRLEPGELVRDSLAHLAMGADAICYFQWRQSKAGAEKWHSAMVPHAGPDSQIFRDVCELGADLNKLADEG
LLSTKLVKSKVAIVFDYESQWATEHTATPTQEVRHWTEPLDWFRALADNGLTADVVPVRGPWDEYEAVVLPSLAILSEQT
TRRVREYVANGGKLFVTYYTGLVDDRDHVWLGGYPGSIRDVVGVRVEEFAPMGTDAPGTMDHLDLDNGTVAHDFADVITS
VADTAHVVASFKADKWTGFDGAPAITVNDFGDGKAAYVGARLGREGLAKSLPALLEELGIETSAEDDRGEVLRVERADET
GENHFVFLFNRTHDVAVVDVEGEPLVASLAQVNESEHTAAIQPNGVLVVKL