LecA from Pseudomonas aeruginosa in complex with tolcapone (CAS: 134308-13-7)

LecA was produced as a recombinant protein in E. coli without methionine at position 1.

AWKGEVLANNEAGQVTSIIYNPGDVITIVAAGWASYGPTQKWGPQGDREHPDQGLICHDAFCGALVMKIGNSGTIPVNTG
LFRWVAPNNVQGAITLIYNDVPGTYGNNSGSFSVNIGKDQS

24% PEG 2kMME, 0.1 M KSCN, 0.1 M sodium acetate pH 4.5 cocrystallised with tolcapone.

the asymmetric unit contains a dimer, which forms a tetramer with symmetry mates.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

lectin, glycomimetics, Pseudomonas aeruginosa, SUGAR BINDING PROTEIN