Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.5mM Mn2+

The structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS.

horizontal bounce Si(111) double crystal monochromator

Mouse Cyclic GMP-AMP synthase catalytic domain 147-507; three extra residues at the N terminus (G from TEV site, TG from AgeI site). Flexible residues in the coordinate were deleted.

cGAMP synthase,cGAS,m-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1

GTGPDKLKKVLDKLRLKRKDISEAAETVNKVVERLLRRMQKRESEFKGVEQLNTGSYYEHVKISAPNEFDVMFKLEVPRI
ELQEYYETGAFYLVKFKRIPRGNPLSHFLEGEVLSATKMLSKFRKIIKEEVKEIKDIDVSVEKEKPGSPAVTLLIRNPEE
ISVDIILALESKGSWPISTKEGLPIQGWLGTKVRTNLRREPFYLVPKNAKDGNSFQGETWRLSFSHTEKYILNNHGIEKT
CCESSGAKCCRKECLKLMKYLLEQLKKEFQELDAFCSYHVKTAIFHMWTQDPQDSQWDPRNLSSCFDKLLAFFLECLRTE
KLDHYFIPKFNLFSQELIDRKSKEFLSKKIEYERNNGFPIFDKL

His*6-MBP-Tev-AgeI-mcGAS CAT, Kanamycin resistance

0.2 M ammonium acetate, 32% MPD, with 0.1 M Bis-Tris pH 6.5

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

( CHAIN A AND ( RESID 148:506 OR RESID 602:602 ) )

( CHAIN C AND ( RESID 148:506 OR RESID 602:602 ) )

Transferase-DNA complex, cGAS, ATP and divalent metal ion, IMMUNE SYSTEM

PDKLKKVLDKLRLKRKDISEAAETVNKVVERLLRRMQKRESEFKGVEQLNTGSYYEHVKISAPNEFDVMFKLEVPRIELQ
EYYETGAFYLVKFKRIPRGNPLSHFLEGEVLSATKMLSKFRKIIKEEVKEIKDIDVSVEKEKPGSPAVTLLIRNPEEISV
DIILALESKGSWPISTKEGLPIQGWLGTKVRTNLRREPFYLVPKNAKDGNSFQGETWRLSFSHTEKYILNNHGIEKTCCE
SSGAKCCRKECLKLMKYLLEQLKKEFQELDAFCSYHVKTAIFHMWTQDPQDSQWDPRNLSSCFDKLLAFFLECLRTEKLD
HYFIPKFNLFSQELIDRKSKEFLSKKIEYERNNGFPIFDKL