Crystal Structure of FosB from Bacillus cereus with Zinc and (1-hydroxy-2-methylpropyl)phosphonic acid

[(1R)-1-hydroxy-2-methylpropyl]phosphonic acid

Identification and analysis of small molecule inhibitors of FosB from Staphylococcus aureus.

MLNGINHLCFSVSNLEDSIEFYEKVLEGELLVRGRKLAYFNICGVWVALNEEIHIPRNEIYQSYTHIAFSVEQKDFESLL
QRLEENDVHILKGRERDVRDCESIYFVDPDGHKFEFHSGTLQDRLNYYREDKPHMTFY

Protein solution (10 mg/mL FosB in 20 mM HEPES buffer, pH 7.5 and 5 mM inhibitor) and reservoir solution (50 mM Mg formate and 16% (w/v) PEG 3350) were mixed in a Hampton Research VDX plate with a 1:1 ratio of each solution. Cryoprotected in its reservoir solution with 20% glycerol added prior to rapid freezing in liquid nitrogen.

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Hydrogens have been added in their riding positions

FosB, inhibitor, homodimer, bacillithiol-S-transferase, TRANSFERASE