Cryo-EM structure of a Zinc-loaded D51A mutant of the YiiP-Fab complex

Characterization of individual Zn2+ binding sites of YiiP

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)

MTQTSQYDFWVKLASRASVATALTLITIKLLAWLYSGSASMLASLTDSFAATLASIINFIAIRYAIVPADHDHRYGHGKA
EPLAALAQSAFIMGSAFLLLFYGGERLLNPSPVENATLGVVVSVVAIVLTLALVLLQKRALAATNSTVVEADSLHYKSDL
FLNAAVLLALVLSQYGWWWADGLFAVLIACYIGQQAFDLGYRSIQALLDRELDEDTRQRIKLIAKEDPRVLGLHDLRTRQ
AGKTVFIQFHLELDGNLSLNEAHSITDTTGLRVKAAFEDAEVIIHQDPVQVEPTTQ

SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQ
PEDFATYYCQQIWSWPLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGN
SQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

EISEVQLVESGGGLVQPGGSLRLSCAASGFTIYSSSIHWVRQAPGKGLEWVASIYSSSGSTYYADSVKGRFTISADTSKN
TAYLQMNSLRAEDTAVYYCARQSYSGLSPRRHWSYGAMDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVK
DYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Zinc transporter, cation diffusion facilitator, membrane protein, transport protein

MTQTSQYDFWVKLASRASVATALTLITIKLLAWLYSGSASMLASLTDSFADTLASIINFIAIRYAIVPADHDHRYGHGKA
EPLAALAQSAFIMGSAFLLLFYGGERLLNPSPVENATLGVVVSVVAIVLTLALVLLQKRALAATNSTVVEADSLHYKSDL
FLNAAVLLALVLSQYGWWWADGLFAVLIACYIGQQAFDLGYRSIQALLDRELDEDTRQRIKLIAKEDPRVLGLHDLRTRQ
AGKTVFIQFHLELDGNLSLNEAHSITDTTGLRVKAAFEDAEVIIHQDPVQVEPTTQ