Anti-CRISPR protein AcrIIC5 inhibits CRISPR-Cas9 by acting as a DNA mimic
Anti-CRISPR Protein AcrIIC5 Inhibits CRISPR-Cas9 by Occupying the Target DNA Binding Pocket.
The dataset used is selenium labelled hence Met 87 is labelled as MSE
(MSE)TIKEDG(MSE)SETQYFVSHDGNRHDLFDTLEQAEHYILKKNGWTDGEIAEKWAFVKKEARKYGGDPFSSNGRHS LWFITELKLSDGVI(MSE)EVDGQLFDDYVESISAERGTEEFAETKRRLVGYYLGW
MTIKEDGMSETQYFVSHDGNRHDLFDTLEQAEHYILKKNGWTDGEIAEKWAFVKKEARKYGGDPFSSNGRHSLWFITELK LSDGVIMEVDGQLFDDYVESISAERGTEEFAETKRRLVGYYLGW
0.1M Tris pH 8.5 and 2.0M ammonium sulfate
Natural Sciences and Engineering Research Council (NSERC, Canada)
Canadian Institutes of Health Research (CIHR)
Anti-CRISPR, CRISPR-Cas, Cas9, inhibition, PROTEIN BINDING