Crystal structure of cyanophycin dipeptide hydrolase CphZ E251A from Acinetobacter baylyi DSM587 in complex with beta-Asp-Arg

(2~{S})-4-[[(2~{S})-5-[[azanyl($l^{4}-azanylidene)methyl]amino]-1-$l^{1}-oxidanyl-1-oxidanylidene-pentan-2-yl]amino]-2-$l^{2}-azanyl-4-oxidanylidene-butanoic acid

Discovery of cyanophycin dipeptide hydrolase enzymes suggests widespread utility of the natural biopolymer cyanophycin.

TSACENFLLPADQDGIQRQVTIFRYGQENSAPKAYLQAGLHADEFPGMLALKYLRDLLDEAARRNRIKGEIVIIPQANPI
GLSQWKDGFLLGRFDHQTGTNFNRDYPDLCQLTVEKLDGQLTENAEHNIDVIRKTMRSALSELKPEQAVDVLRHKLISES
CDADLVLDLHADNQAQCHMYTLTPLWPAMHDVAAEIDARAVLLAEESGGHPFDEACSAPWMNLSRAFPDYPIPLACQSAT
FALGSNDEVDLRLAQDQAEALFRILIRRGFIEDVHVGELPQLACEGTLLEAMQQLKAPCQGLIVYHNRLGDFVRSGDKVV
SIVDPIGETVDILAHTDGVLFARHSQTYAYPNKVIGKIAGKEPLPERKGF

0.1 M bis-tris propane pH 7.5, 24% PEG3350, 0.2 M NaBr, 10 mM spermine and 10 mM LiCl

Canadian Institutes of Health Research (CIHR)

TSACENFLLPADQDGIQRQVTIFRYGQENSAPKAYLQAGLHADEFPGMLALKYLRDLLDEAARRNRIKGEIVIIPQANPI
GLSQWKDGFLLGRFDHQTGTNFNRDYPDLCQLTVEKLDGQLTENAEHNIDVIRKTMRSALSELKPEQAVDVLRHKLISES
CDADLVLDLHADNQAQCHMYTLTPLWPAMHDVAAEIDARAVLLAEESGGHPFDEACSAPWMNLSRAFPDYPIPLACQSAT
FELGSNDEVDLRLAQDQAEALFRILIRRGFIEDVHVGELPQLACEGTLLEAMQQLKAPCQGLIVYHNRLGDFVRSGDKVV
SIVDPIGETVDILAHTDGVLFARHSQTYAYPNKVIGKIAGKEPLPERKGF