Crystal structure of the WWP2 HECT domain in complex with H301, a Helicon Polypeptide

Recognition and reprogramming of E3 ubiquitin ligase surfaces by alpha-helical peptides.

NEDD4-like E3 ubiquitin-protein ligase WWP2

Atrophin-1-interacting protein 2,AIP2,HECT-type E3 ubiquitin transferase WWP2,WW domain-containing protein 2

GPDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSH
EVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDP
EFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFN
EVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGF
AELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETE

0.1M Sodium acetate, 0.1M sodium citrate pH6.0, 10% PEG4000

E3 ligase, complex, stapled peptide, LIGASE

(chain A and (resid 493 through 500 or resid 502 through 599 or resid 601 through 605 or resid 607 through 672 or resid 674 or resid 676 through 699 or resid 701 through 770 or resid 772 through 805 or resid 808 through 818 or resid 820 through 865))

(chain B and (resid 493 through 500 or resid 502 through 599 or resid 601 through 605 or resid 607 through 672 or resid 674 or resid 676 through 699 or resid 701 through 770 or resid 772 through 805 or resid 808 through 818 or resid 820 through 865))

(chain C and (resid 493 through 500 or resid 502 through 599 or resid 601 through 605 or resid 607 through 672 or resid 674 or resid 676 through 699 or resid 701 through 770 or resid 772 through 805 or resid 808 through 818 or resid 820 through 865))

(chain D and (resid 493 through 500 or resid 502 through 599 or resid 601 through 605 or resid 607 through 672 or resid 674 or resid 676 through 699 or resid 701 through 770 or resid 772 through 805 or resid 808 through 818 or resid 820 through 865))

(chain E and (resid 7 through 17 or resid 103))

(chain F and (resid 7 through 17 or resid 103))

DRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEV
LNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEF
YNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEV
APLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAE
LIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETE