A structural dendrogram of the actinobacteriophage major capsid proteins provides important structural insights into the evolution of capsid stability.

Amino acid sequence built into the map for a single major capsid protein and refined with Phenix. Model then used for rest of asymmetric unit and refined with Phenix. Final step involved using Isolde.

Standard CTF correction inside RELION's reconstruction.

MATGRTTEGRTVTVTTASNTTITGAAGTFVASDVGRTITRAGIPAGTTITAVASGTSATISAAATTSATSAATLGSLNGQ
SQGLVGWSPETDTEAGAYSVAATNAGTVTPDRLTNAFTPVSQRGRG

MGIYRKKDVIPIPYNNLTSRTDAQALIPEEVSKEMLGKATEQSAVLQQFRRIPVGRNQVRFPVLSALPVAYWVNGDTGLK
QTTEINWTNKYLNIEEIATIVPVPDNVLADVDVNVWDEAMPYLIEAFGRTLDSAVVFGTNAPGTFPQAIVPAIAAAGNAV
TEGSTAAQGGYLGDLDKAYGKVEEDGFEANGIIAGIGLKGKLRGARDANGQKIDPTRVGADLKSIDGLPVSYPMRGLWPS
AGGAGTNVRALVGDWSQFVVGVRQDITLKVLDQAVIQDNTGAIIYNLAQQDMTALRLTFRVGWQVANTLNNDQPTEANRY
PAASLIF

icosahedral asymmetric unit, std point frame

HK97-fold, T=7, tailed bacteriophage, VIRUS