Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2)

Coupled catalytic states and the role of metal coordination in Cas9.

CryoEM Structure of AceCas9 (Cleavage intermediate 2)

CRISPR-associated endonuclease, Csn1 family/RNA

CRISPR-associated endonuclease, Csn1 family

DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3')

DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3')

DNA non-target strand (5'-D(P*TP*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3')

DNA non-target strand (5'-D(P*AP*GP*A)-3')

MGGSEVGTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERSGASRLALRGMARRARRLRRFRRAR
LRDLDMLLSELGWTPLPDKNVSPVDAWLARKRLAEEYVVDETERRRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQPSDS
WERTRESLEARYSVSLEPGTVGQWAGYLLQRAPGIRLNPTQQSAGRRAELSNATAFETRLRQEDVLWELRCIADVQGLPE
DVVSNVIDAVFCQKRPSVPAERIGRDPLDPSQLRASRACLEFQEYRIVAAVANLRIRDGSGSRPLSLEERNAVIEALLAQ
TERSLTWSDIALEILKLPNESDLTSVPEEDGPSSLAYSQFAPFDETSARIAEFIAKNRRKIPTFAQWWQEQDRTSRSDLV
AALADNSIAGEEEQELLVHLPDAELEALEGLALPSGRVAYSRLTLSGLTRVMRDDGVDVHNARKTCFGVDDNWRPPLPAL
HEATGHPVVDRNLAILRKFLSSATMRWGPPQSIVVELARGASESRERQAEEEAARRAHRKANDRIRAELRASGLSDPSPA
DLVRARLLELYDCHCMYCGAPISWENSELDHIVPRTDGGSNRHENLAITCGACNKEKGRRPFASWAETSNRVQLRDVIDR
VQKLKYSGNMYWTRDEFSRYKKSVVARLKRRTSDPEVIQSIESTGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARR
WLDISIERLFSRVAIFAQSTSTKRLDRRHHAVDAVVLTTLTPGVAKTLADARSRRVSAEFWRRPSDVNRHSTEEPQSPAY
RQWKESCSGLGDLLISTAARDSIAVAAPLRLRPTGALHEETLRAFSEHTVGAAWKGAELRRIVEPEVYAAFLALTDPGGR
FLKVSPSEDVLPADENRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGAAYIASFHHARVFRWGSSHSPSFALLRVSLADL
AVAGLLRDGVDVFTAELPPWTPAWRYASIALVKAVESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSMFLKYFPERHWVV
TGFEDDKRINLKPAFLSAEQAEVLRTERSDRPDTLTEAGEILAQFFPRCWRATVAKVLCHPGLTVIRRTALGQPRWRRGH
LPYSWRPWSADPWSGGTP

(GTP)GUAGGAUGGCAAGAUCCUGGUAUGCUGGGGAGCCUGAAAAGGCUACCUAGCAAGACCCCUUCGUGGGGUCGCAUU
CUUCACCCCCUCGCAGCAGCGAGGGGGUUC

GGUAGGAUGGCAAGAUCCUGGUAUGCUGGGGAGCCUGAAAAGGCUACCUAGCAAGACCCCUUCGUGGGGUCGCAUUCUUC
ACCCCCUCGCAGCAGCGAGGGGGUUC

(DC)(DC)(DA)(DG)(DG)(DA)(DT)(DC)(DT)(DT)(DG)(DC)(DC)(DA)(DT)(DC)(DC)(DT)(DA)(DC)
(DC)(DT)(DC)(DT)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Cas9, AcCas9, Crispr, RNA BINDING PROTEIN, RNA BINDING PROTEIN-DNA-RNA complex