Cryo EM structure of the vaccinia complete RNA polymerase complex lacking the capping enzyme

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes.

Vaccinia complete RNA polymerase complex lacking the capping enzyme

DNA-directed RNA polymerase 133 kDa polypeptide

DNA-directed RNA polymerase 22 kDa subunit

DNA-directed RNA polymerase 19 kDa subunit

DNA-directed RNA polymerase 18 kDa subunit

RNA polymerase-associated transcription-specificity factor RAP94

DNA-directed RNA polymerase 7 kDa subunit

Early transcription factor 82 kDa subunit

DNA-directed RNA polymerase 147 kDa polypeptide

DNA-directed RNA polymerase 35 kDa subunit

DNA-directed RNA polymerase 30 kDa polypeptide

Protein H4,RPO-associated protein of 94 kDa

ETF large subunit,VETF A7 subunit,Vaccinia virus early transcription factor large subunit,VETF large subunit

Factor X,NPH-I,Nucleoside triphosphate phosphohydrolase I,NPH I

MKKNTNSEMDQRLGYKFLVPDPKAGVFYRPLHFQYVSYSNFILHRLHEILTVKRPLLSFKNNTERIMIEISNVKVTPPDY
SPIIASIKGKSYDALATFTVNIFKEVMTKEGISITKISSYEGKDSHLIKIPLLIGYGNKNPLDTAKYLVPNVIGGVFINK
QSVEKVGINLVEKITTWPKFRVVKPNSFTFSFSSVSPPNVLPTRYRHYKISLDISQLEALNISSTKTFITVNIVLLSQYL
SRVSLEFIRRSLSYDMPPEVVYLVNAIIDSAKRITESITDFNIDTYINDLVEAEHIKQKSQLTINEFKYEMLHNFLPHMN
YTPDQLKGFYMISLLRKFLYCIFHTSRYPDRDSMVCHRILTYGKYFETLAHDELENYIGNIRNDIMNNHKNRGTYAVNIH
VLTTPGLNHAFSSLLSGKFKKSDGSYRTHPHYSWMQNISIPRSVGFYPDQVKISKMFSVRKYHPSQYLYFCSSDVPERGP
QVGLVSQLSVLSSITNILTSEYLDLEKKICEYIRSYYKDDISYFETGFPITIENALVASLNPNMICDFVTDFRRRKRMGF
FGNLEVGITLVRDHMNEIRINIGAGRLVRPFLVVDNGELMMDVCPELESRLDDMTFSDIQKEFPHVIEMVDIEQFTFSNV
CESVQKFRMMSKDERKQYDLCDFPAEFRDGYVASSLVGINHNSGPRAILGCAQAKQAISCLSSDIRNKIDNGIHLMYPER
PIVISKALETSKIAANCFGQHVTIALMSYKGINQEDGIIIKKQFIQRGGLDIVTAKKHQVEIPLENFNNKERDRSNAYSK
LESNGLVRLNAFLESGDAMARNISSRTLEDDFARDNQISFDVSEKYTDMYKSRVERVQVELTDKVKVRVLTMKERRPILG
DKFTTRTSQKGTVAYVADETELPYDENGITPDVIINSTSIFSRKTISMLIEVILTAAYSAKPYNNKGENRPVCFPSSNET
SIDTYMQFAKQCYEHSNPKLSDEELSDKIFCEKILYDPETDKPYASKVFFGPIYYLRLRHLTQDKATVRCRGKKTKLIRQ
ANEGRKRGGGIKFGEMERDCLIAHGAANTITEVLKDSEEDYQDVYVCENCGDIAAQIKGINTCLRCSKLNLSPLLTKIDT
THVSKVFLTQMNARGVKVKLDFERRPPSFYKPLDKVDLKPSFLV

MNQYNVKYLAKILCLKTEIARDPYAVINRNVLLRYTTDIEYNDLVTLITVRHKIDSMKTVFQVFNESSINYTPVDDDYGE
PIIITSYLQKGHNKFPVNFLYIDVVISDLFPSFVRLDTTETNIVNSVLQTGDGKKTLRLPKMLETEIVVKILYRPNIPLK
IVRFFRNNMVTGVEIADRSVISVAD

MADTDDIIDYESDDLTEYEDDEEEEEDGESLETSDIDPKSSYKIVESASTHIEDAHSNLKHIGNHISALKRRYTRRISLF
EIAGIIAESYNLLQRGRLPLVSEFSDETMKQNMLHVIIQEIEEGSCPIVIEKNGELLSVNDFDKDGLKFHLDYIIKIWKL
QKRY

MSSFVTNGYLSVTLEPHELTLDIKTNIRNAVYKTYLHREISGKMAKKIEIREDVELPLGEIVNNSVVINVPCVITYAYYH
VGDIVRGTLNIEDESNVTIQCGDLICKLSRDSGTVSFSDSKYCFFRNGNAYDNGSEVTAVLMEAQQGIESSFVFLANIVD
S

MDSKETILIEIIPKIKAYLLDANISPKSYDDFISRNKNIFVINLYNVSTITEEDIRLLYTTIEQNIDADDQTLVAIFSYI
GYKFEQAVKEEISTSLSFNDKNTTDEMTYNLYDLFFNTLDMYLRQKKISILVNDDVRGDVIVSYKNSDLVSSFNAELEPE
IKKIPFNMKNLLPYLEKNLDQLRFSKKYLDFAYLCRHIGIPISKKKYNVRYVFLYKIDGLSIPIIIKDFLDVKYVYLENT
GKIYKNSFSEDHNNSLSDWGKVIIPLLKDRHLYSYIFLSSYHLHSYYTDLIARDEPVFVKRKKLDIIEIDEPEAWKRDVR
VEFAPCEHQIRLKEAMKVDANYFTKINNFANEFIYYEDGVAYCRVCGINIPIFNLDAADVIKNTVIVSTFNKTIFLSEPY
SYFVHSQRFIFNIIMSFDNIMKSQTWVMKYNINRLILNFLIDINSRRQEYEKKFSSEIKRGLFFLRLSANLFESQVSSTE
LFYVSKMLNLNYIVALVIILNSSADFIVSYMTSKNKTVEESTLKYAISVVIYDFLVKTRICEKGSLDTIVLFTDVYTSIM
PEELDLHFQRITLELRKLVSIQRSALEPNYDVESRGEELPLSALKFFDTSTIIVKTMAPVHTCVEQKIVAPTPSVEPTDA
SLKNFKELTCDEDIKILIRVHDTNATKLVIFPSHLKIEIERKKLIIPLKSLYITNTLKYYYSNSYLYVFRFGDPMPFEEE
LIDHEHVQYKINCYNILRYHLLPDSDVFVYFSNSLNREALEYAFYIFLSKYVNVKQWIDENITRIKELYMINFNN

MELVNIFLETDAGRVKFAIKNTDDVCASELINKFVELLSEYIHIDQSEFYLVVKDKDIFYFKCDRGSISIVNNEFYVFDE
PLLFVKDFTNVTGVEFIVTETMPCRIIPKNNHAVISVVTNHKFYNGLSL

MRYIVSPQLVLQVGKGQEVERALYLTPYDYIDEKSPIYYFLRSHLNIQQPEIVKRHILLTLRMTQLKGYLGNLLDIKDDI
IIYSHKNNLEYSYVDNTIFNPFVYTQKKTLLKNDSFLYNVYPGACDFLVIWVARACDTSIPEFGSYEDVDNNIIKFETML
MEVFPQLDLDITVESKFNNIFRTNLKLTGLKKIIQRVQDLDINYKSLLSRYDEHFINMTGNHFILNDEQLNLSIWDLDGT
LALSSDGDTVMINNVKLFTDLVSDIDTQMERIKGDITYKVHLATPINSRIKLDIETSFIFIETATNNILLSSDKKISIIL
AKNHISIKVKNHIPNIEKYFTFLVIAINAMFNSVQKSADFTKVETVYWSRICQNTKNKNRKPIIINYLDPGMKKISNNFY
RSDEKEVFINDNGIMFTCMDPLGKYNKVGFLNIFHDMRKYCIPCCFLHDQSHRSTFSSCVHQIDVEKKIVSPYILNFGKV
VTESKMSFLPIIFDAFLNDGMTANMEQDNKRLKETSGYHIVRCCAGDDIVRLRTTSDIIQFVNEDKNILIVNDMVYFPMN
ASDIGKKIHILIQEIVHEVMIVKKKESSDKIDFFPPNYKLLKDLFPKQTIQTPIQSDAGMVLTTDGFYIDGKLFNEDLSS
KYVTFTKNVIASDAVAKYFSPLFKYVISEAKDRFIKTWMINIMIHMNVDPNNIIPTLEKYYPNSGRAQIN

MAVISKVTYSLYDQKEINATDIIISHVKNDDDIGTVKDGRLGAMDGALCKTCGKTELECFGHWGKVSIYKTHIVKPEFIS
EIIRLLNHICIHCGLLRSREPYSDDINLKELSGHALRRLKDKILSKKKSCWNSECMQPYQKITFSKKKVCFVNKLDDINV
PNSLIYQKLISIHEKFWPLLEIHQYPANLFYTDYFPIPPLIIRPAISFWIDSIPKETNELTYLLGMIVKNCNLNADEQVI
QKAVIEYDDIKIISNNTTSINLSYITSGKNNMIRSYIVARRKDQTARSVIGPSTSITVNEVGMPAYIRNTLTEKIFVNAF
TVDKVKQLLASNQVKFYFNKRLNQLTRIRQGKFIKNKIHLLPGDWVEVAVQEYTSIIFGRQPSLHRYNVIASSIRATEGD
TIKISPGIANSQNADFDGDEEWMILEQNPKAVIEQSILMYPTTLLKHDIHGAPVYGSIQDEIVAAYSLFRIQDLCLDEVL
NILGKYGREFDPKGKCKFSGKDIYTYLIGEKINYPGLLKDGEIIANDVDSNFVVAMRHLSLAGLLSDHKSNVEGINFIIK
SSYVFKRYLSIYGFGVTFKDLRPNSTFTNKLEAINVEKIELIKEAYAKYLNDVRDGKIVPLSKALEADYVESMLSNLTNL
NIREIEEHMRQTLIDDPDNNLLKMAKAGYKVNPTELMYILGTYGQQRIDGEPAETRVLGRVLPYYLPDSKDPEGRGYILN
SLTKGLTGSQYYFSMLVARSQSTDIVCETSRTGTLARKIIKKMEDMVVDGYGQVVIGNTLIKYAANYTKILGSVCKPVDL
IYPDESMTWYLEISALWNKIKQGFVYSQKQKLAKKTLAPFNFLVFVKPTTEDNAIKVKDLYDMIHNVIDDVREKYFFTVS
NIDFMEYIFLTHLNPSRIRITKETAITIFEKFYEKLNYTLGGGTPIGIISAQVLSEKFTQQALSSFHTTEKSGAVKQKLG
FNEFNNLTNLSKNKTEIITLVSDDISKLQSVKINFEFVCLGELNPNITLRKETDKYVVDIIVNRLYIKRAEITELVVEYM
IERFISFSVIVKEWGMETFIEDEDNIRFTVYLNFVEPEELNLSKFMMVLPGAANKGKISKFKIPISDYTGYDDFNQTKKL
NKMTVELMNLKELGSFDLENVNVYPGVWNTYDIFGIEAAREYLCEAMLNTYGEGFDYLYQPCDLLASLLCASYEPESVNK
FKFGAASTLKRATFGDNKALLNAALHKKSEPINDNSSCHFFSKVPNIGTGYYKYFIDLGLLMRMERKLSDKISSQKIKEM
EETEDF

MSKSHAAYIDYALRRTTNMPVEMMGSDVVRLKDYQHFVARVFLGLDSMHSLLLFHETGVGKTMTTVYILKHLKDIYTNWA
IILLVKKALIEDPWMNTILRYAPEITKDCIFINYDDQNFRNKFFTNIKTINSKSRICVIIDECHNFISKSLIKEDGKIRP
TRSVYNFLSKTIALKNHKMICLSATPIVNSVQEFTMLVNLLRPGSLQHQSLFENKRLVDEKELVSKLGGLCSYIVNNEFS
IFDDVEGSASFAKKTVLMRYVNMSKKQEEIYQKAKLAEIKTGISSFRILRRMATTFTFDSFPERQNRDPGEYAQEIATLY
NDFKNSLRDREFSKSALDTFKKGELLKGDASAADISLFTELKEKSVKFIDVCLGILASHGKCLVFEPFVNQSGIEILLLY
FKVFGISNIEFSSRTKDTRIKAVAEFNQESNTNGECIKTCVFSSSGGEGISFFSINDIFILDMTWNEASLRQIVGRAIRL
NSHVLTPPERRYVNVHFIMARLSNGMPTVDEDLFEIIQSKSKEFVQLFRVFKHTSLEWIHANEKDFSPIDNESGWKTLVS
RAIDLSSKKNITNKLIEGTNIWYSNSNRLMSINRGFKGVDGRVYDVDGNYLHDMPDNPVIKIHDGKLIYIF

MQHPREENSIVVELEPSLATFIKQGFNNLVKWPLLNIGIVLSNTSTAVNEEWLTAVEHIPTMKIFYKHIHKILTREMGFL
VYLKRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIELIAFSSGTVINEDIVSKLTF
LDVEVFNREYNNVKTIIDPDFVFRSPFIVISPMGKLTFFVEVYSWFDFKSCFKDIIDFLEGALIANIHNHMIKVGNCDET
VSSYNPESGMLFVNDLMTMNIVNFFGCNSRLESYHRFDMTKVDVELFIKALSDACKKILSASNRL

MENVYISSYSSNEQTSMAVAATDIRELLSQYVDDANLEDLIEWAMEKSSKYYIKNIGNTKSNIEETKFESKNNIGIEYSK
DSRNKLSYRNKPSIATNLEYKTLCDMIKGTSGTEKEFLRYLLFGIKCIKKGVEYNIDKIKDVSYNDYFNVLDEKYNTPCP
NCKSRNTTPMMIQTRAADEPPLVRHACRDCKQHFKPPKFRAFRNLNVTTQSIHENKEITEILPDNNP(SEP)PPE(SEP)
PEPA(SEP)PIDDGLIRATFDRNDEPPEDDE

MENVYISSYSSNEQTSMAVAATDIRELLSQYVDDANLEDLIEWAMEKSSKYYIKNIGNTKSNIEETKFESKNNIGIEYSK
DSRNKLSYRNKPSIATNLEYKTLCDMIKGTSGTEKEFLRYLLFGIKCIKKGVEYNIDKIKDVSYNDYFNVLDEKYNTPCP
NCKSRNTTPMMIQTRAADEPPLVRHACRDCKQHFKPPKFRAFRNLNVTTQSIHENKEITEILPDNNPSPPESPEPASPID
DGLIRATFDRNDEPPEDDE

RNA polymerase, RNAP, DNA-dependent RNA polymerase, VIRAL PROTEIN

MKKNTDSEMDQRLGYKFLVPDPKAGVFYRPLHFQYVSYSNFILHRLHEILTVKRPLLSFKNNTERIMIEISNVKVTPPDY
SPIIASIKGKSYDALATFTVNIFKEVMTKEGISITKISSYEGKDSHLIKIPLLIGYGNKNPLDTAKYLVPNVIGGVFINK
QSVEKVGINLVEKITTWPKFRVVKPNSFTFSFSSVSPPNVLPTRYRHYKISLDISQLEALNISSTKTFITVNIVLLSQYL
SRVSLEFIRRSLSYDMPPEVVYLVNAIIDSAKRITESITDFNIDTYINDLVEAEHIKQKSQLTINEFKYEMLHNFLPHMN
YTPDQLKGFYMISLLRKFLYCIYHTSRYPDRDSMVCHRILTYGKYFETLAHDELENYIGNIRNDIMNNHKNRGTYAVNIH
VLTTPGLNHAFSSLLSGKFKKSDGSYRTHPHYSWMQNISIPRSVGFYPDQVKISKMFSVRKYHPSQYLYFCSSDVPERGP
QVGLVSQLSVLSSITNILTSEYLDLEKKICEYIRSYYKDDISYFETGFPITIENALVASLNPNMICDFVTDFRRRKRMGF
FGNLEVGITLVRDHMNEIRINIGAGRLVRPFLVVDNGELMMDVCPELESRLDDMTFSDIQKEFPHVIEMVDIEQFTFSNV
CESVQKFRMMSKDERKQYDLCDFPAEFRDGYVASSLVGINHNSGPRAILGCAQAKQAISCLSSDIRNKIDNGIHLMYPER
PIVISKALETSKIAANCFGQHVTIALMSYKGINQEDGIIIKKQFIQRGGLDIVTAKKHQVEIPLENFNNKERDRSNAYSK
LESNGLVRLNAFLESGDAMARNISSRTLEDDFARDNQISFDVSEKYTDMYKSRVERVQVELTDKVKVRVLTMKERRPILG
DKFTTRTSQKGTVAYVADETELPYDENGITPDVIINSTSIFSRKTISMLIEVILTAAYSAKPYNNKGENRPVCFPSSNET
SIDTYMQFAKQCYEHSNPKLSDEELSDKIFCEKILYDPETDKPYASKVFFGPIYYLRLRHLTQDKATVRCRGKKTKLIRQ
ANEGRKRGGGIKFGEMERDCLIAHGAANTITEVLKDSEEDYQDVYVCENCGDIAAQIKGINTCLRCSKLNLSPLLTKIDT
THVSKVFLTQMNARGVKVKLDFERRPPSFYKPLDKVDLKPSFLV

MSSFVTNGYLPVTLEPHELTLDIKTNIRNAVYKTYLHREISGKMAKKIEIREDVELPLGEIVNNSVVINVPCVITYAYYH
VGDIVRGTLNIEDESNVTIQCGDLICKLSRDSGTVSFSDSKYCFFRNGNAYDNGSEVTAVLMEAQQGIESSFVFLANIVD
S

MDSKETILIEIIPKIKAYLLDANISPKSYDDFISRNKNIFVINLYNVSTITEEDIRLLYTTIEQNIDADDQTLVAIFSYI
GYKFEQAVKEEISTSLSFNDKNTTDEMTYNLYDLFFNTLDMYLRQKKISILVNDDVRGDVIVSYKNSDLVSSFNAELEPE
IKKIPFNMKNLLPYLEKNLDQLRFSKKYLDFAYLCRHIGIPISKKKYNVRYVFLYKIDGLSIPIIIKDFLDVKYVYLENT
GKIYKNSFSEDHNNSLSDWGKVIIPLLKDRHLYSYIFLSSYHLHSYYTDLIARDEPVFVKRKKLDIIEIDEPEAWKRDVR
VEFAPCEHQIRLKEAMKVDANYFTKINNFANEFIYYEDGVAYCRVCGINIPIFNLDAADVIKNTVIVSTFNKTIFLSEPY
SYFVHSQRFIFNIIMSFDNIMKSQTWVMKYNINRLILNFLIDINSRRQEYEKKFSSEIKRGLFFLRLSANLFESQVSSTE
LFYVSKMLNLNYIVALVIILNSSADFIVSYMTSKNKTVEESTLKYAISVVIYDFLVKTRICEKGSLDTIVLFTDVYTSIM
PEELDLHFQRITLELRKLVSIQRSALEPNYDVESRGEELPLSALKFFDTSTIIVKTMAPVHTYIEQKIVAPTPSVEPTDA
SLKNFKELTCDEDIKILIRVHDTNATKLVIFPSHLKIEIERKKLIIPLKSLYITNTLKYYYSNSYLYVFRFGDPMPFEEE
LIDHEHVQYKINCYNILRYHLLPDSDVFVYFSNSLNREALEYAFYIFLSKYVNVKQWIDENITRIKELYMINFNN

MRYIVSPQLVLQVGKGQEVERALYLTPYDYIDEKSPIYYFLRSHLNIQQPEIVKRHILLTLRMTQLKGYLGNLLDIKDDI
IIYSHKNNLEYSYVDNTIFNPFVYTQKKTLLKNDSFLYNVYPGACDFLVIWVARACDTSIPEFGSYEDVDNNIIKFETML
MEVFPQLDLDITVESKFNNIFRTNLKLTGLKKIIQRVQDLDINYKSLLSRYDEHFINMTGNHFILNDEQLNLSIWDLDGT
LALSSDGDTVMINNVKLFTDLVSDIDTQMERIKGDITYKVHLATPINSRIKLDIETSFIFIETATNNILLSSDKKISIIL
AKNHISIKVKNHIPNIEKYFTFLVIAINAMFNSVQKSADFTKVETVYWSRICQNTKNKNRKPIIINYLDPGMKKISNNFY
KSDEKEVFINDNGIMFTCMDPLGKYNKVGFLNIFHDMRKYCIPCCFLHDQSHRSTFSSCVHQIDVEKKIVSPYILNFGKV
VTESKMSFLPIIFDAFLNDGMTANMEQDNKRLKETSGYHIVRCCAGDDIVRLRTTSDIIQFVNEDKNILIVNDMVYFPMN
ASDIGKKIHILIQEIVHEVMIVKKKESSDKIDFFPPNYKLLKDLFPKQTIQTPIQSDAGMVLTTDGFYIDGKLFNEDLSS
KYVTFTKNVIASDAVAKYFSPLFKYVISEAKDRFIKTWMINIMIHMNVDPNNIIPTLEKYYPNSGRAQIN

MAVISKVTYSLYDQKEINATDIIISHVKNDDDIGTVKDGRLGAMDGALCKTCGKTELECFGHWGKVSIYKTHIVKPEFIS
EIIRLLNHICIHCGLLRSREPYSDDINLKELSGHALRRLKDKILSKKKSCWNSECMQPYQKITFSKKKVCFVNKLDDINV
PNSLIYQKLISIHEKFWPLLEIHQYPANLFYTDYFPIPPLIIRPAISFWIDSIPKETNELTYLLGMIVKNCNLNADEQVI
QKAVIEYDDIKIISNNTSSINLSYITSGKNNMIRSYIVARRKDQTARSVIGPSTSITVNEVGMPAYIRNTLTEKIFVNAF
TVDKVKQLLASNQVKFYFNKRLNQLTRIRQGKFIKNKIHLLPGDWVEVAVQEYTSIIFGRQPSLHRYNVIASSIRATEGD
TIKISPGIANSQNADFDGDEEWMILEQNPKAVIEQSILMYPTTLLKHDIHGAPVYGSIQDEIVAAYSLFRIQDLCLDEVL
NILGKYGREFDPKGKCKFSGKDIYTYLIGEKINYPGLLKDGEIIANDVDSNFVVAMRHLSLAGLLSDHKSNVEGINFIIK
SSYVFKRYLSIYGFGVTFKDLRPNSTFTNKLEAINVEKIELIKEAYAKYLNDVRDGKIVPLSKALEADYVESMLSNLTNL
NIREIEEHMRQTLIDDPDNNLLKMAKAGYKVNPTELMYILGTYGQQRIDGEPAETRVLGRVLPYYLPDSKDPEGRGYILN
SLTKGLTGSQYYFSMLVARSQSTDIVCETSRTGTLARKIIKKMEDMVVDGYGQVVIGNTLIKYAANYTKILGSVCKPVDL
IYPDESMTWYLEISALWNKIKQGFVYSQKQKLAKKTLAPFNFLVFVKPTTEDNAIKVKDLYDMIHNVIDDVREKYFFTVS
NIDFMEYIFLTHLNPSRIRITKETAITIFEKFYEKLNYTLGGGTPIGIISAQVLSEKFTQQALSSFHTTEKSGAVKQKLG
FNEFNNLTNLSKNKTEIITLVSDDISKLQSVKINFEFVCLGELNPDHSLFEKKQDRYVVDIIVNKLYIKRAEITELVVEY
MIERFISFSVIVKEWGMETFIEDEDNIRFTVYLNFVEPEELNLSKFMMVLPGAANKGKISKFKIPISDYTGYDCFNQTKK
LNKMTVELMNLKELGSFDLENVNVYPGVWNTYDIFGIEAAREYLCEAMLNTYGEGFDYLYQPCDLLASLLCASYEPESVN
KFKFGAASTLKRATFGDNKALLNAALHKKSEPINDNSSCHFFSKVPNIGTGYYKYFIDLGLLMRMERKLSDKISSQKIKE
MEETEDF

MSKSHAAYIDYALRRTTNMPVEMMGSDVVRLKDYQHFVARVFLGLDSMHSLLLFHETGVGKTMTTVYILKHLKDIYTNWA
IILLVKKALIEDPWMNTILRYAPEITKDCIFINYDDQNFRNKFFTNIKTINSKSRICVIIDECHNFISKSLIKEDGKIRP
TRSVYNFLSKTIALKNHKMICLSATPIVNSVQEFTMLVNLLRPGSLQHQSLFENKRLVDEKELVSKLGGLCSYIVNNEFS
IFDDVEGSASFAKKTVLMRYVNMSKKQEEIYQKAKLAEIKTGISSFRILRRMATTFTFDSFPERQNRDPGEYAQEIATLY
NDFKNSLRDREFSKSALDTFKRGELLGGDASAADISLFTELKEKSVKFIDVCLGILASHGKCLVFEPFVNQSGIEILLLY
FKVFGISNIEFSSRTKDTRIKAVAEFNQESNTNGECIKTCVFSSSGGEGISFFSINDIFILDMTWNEASLRQIVGRAIRL
NSHVLTPPERRYVNVHFIMARLSNGMPTVDEDLFEIIQSKSKEFVQLFRVFKHTSLEWIHANEKDFSPIDNESGWKTLVS
RAIDLSSNKNITNKLIEGTNIWYSNSNRLMSINRGFKGVDGRVYDVDGNYLHDMPDNPVIKIHDGKLIYIF

MQHPREENSIVVELEPSLATFIKQGFNNLVKWPLLNIGIVLSNTSTAVNEEWLTAVEHIPTMKIFYKHIHKILTREMGFL
VYLKRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIELIAFSSGTVINEDIVSKLTF
LDVEVFNREYNNVKTIIDPDFVFRSPFIVISPMGKLTFFVEVYSWFDFKSCLKDIIDFLEGALIANIHNHMIKVGNCDET
VSSYNPESGMLFVNDLMTMNIVNFFGCNSRLESYHRFDMTKVDVELFIKALSDACKKILSASNRL

MENVYISSYSSNEQTSMAVTATDIRELLSQYVDDANLEDLIEWAMEKSSKYYIKNIGNTKSNIEETKFESKNNIGIEYSK
DSRNKLSYRNKPSIATNLEYKTLCDMIKGTSGTEKEFLRYLLFGIKCIKKGVEYNIDKIKDVSYNDYFNVLDEKYNTPCP
NCKSRNTTPMMIQTRAADEPPLVRHACRDCKQHFKPPKFRAFRNLNVTTQSIHENKEITEILPDNNPSPPESPEPASPID
DGLIRATFDRNDEPPEDDE