A redox switch allows binding of Fe(II) and Fe(III) ions in the cyanobacterial iron-binding protein FutA from Prochlorococcus.

A redox switch allows binding of Fe(II) and Fe(III) ions in the cyanobacterial iron binding protein FutA from Prochlorococcus;

Putative iron ABC transporter, substrate binding protein

TEKEVKVYSGRHYNTDKEVYQKFQEQTGIKVRYIETDGKAIIERLKREGKNSQADLVILVDAAIIENASKANLFQKINSN
FLENSVPNNLRDPRNKWFGLTRRLRVIISNPDIVDITKIKNFEDLTNPSFKGKVCLRNRKSPYNQSLVSNQIAKKGVGQT
KIWLKGLISNVSTPYFSGDSSLIRAVGLGTCGIGIVNHYYVARMLDGVKGPRDASLAEKIKLIIPNPAHVNITAGGVYKY
AENKTEAIKLLEYLASSEGSQGLANKTYEHPLKESSQNRIVSKFGDFTPDNVTIKELGKFNSKAIEIMKEVGWN

Prochlorococcus marinus subsp. pastoris str. CCMP1986

Purified protein, diluted crystal seeds, and crystallisation buffer were mixed at a 1:1.5:1.5 ratio. Crystallisation buffer was 25% PEG 3350, 200 mM sodium thiocyanate. Crystal seed stock was diluted 1:20 in 25% PEG 3350.

Hydrogens have been added in their riding positions

Periplasmic iron-binding protein, metal ion binding, iron homeostasis, METAL BINDING PROTEIN