FimH lectin domain in complex with oligomannose-6

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Structural insights into a cooperative switch between one and two FimH bacterial adhesins binding pauci- and high-mannose type N-glycan receptors.

Intervening with urinary tract infections using anti-adhesives based on the crystal structure of the FimH-oligomannose-3 complex.

Sites for Dynamic Protein-Carbohydrate Interactions of O- and C-Linked Mannosides on the E. coli FimH Adhesin.

A Novel Integrated Way for Deciphering the Glycan Code for the FimH Lectin.

PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)

Type 1 fimbrin D-mannose specific adhesin

FACKTANGTAIPIGGGSANVYVNLAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGS
SYPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNSDDFQFVWNIYANNDVVVPT

small lentil-like crystals grown on a large beam

1 M Lithium sulphate
100 mM Tris-HCl, pH 8.5
10 mM Nickel chloride
3% glycerol

H2020 Marie Curie Actions of the European Commission

7BHD contains FimH in monovalent binding with an alpha1,6-core fucosylated N-glycan

Sauer, M. M., Jakob, R. P., Luber, T., Canonica, F., Navarra, G., Ernst, B., Unverzagt, C., Maier, T., and Glockshuber, R. (2019) Binding of the Bacterial Adhesin FimH to Its Natural, Multivalent High-Mannose Type Glycan Targets. J Am Chem Soc 141, 936-944

Hydrogens have been added in their riding positions

Type-1 fimbriae, Escherichia coli, FimH, Adhesin, Lectin, Oligomannose, High-mannose, CELL ADHESION

Local NCS retraints between domains: 1 2

Local NCS retraints between domains: 3 4

Local NCS retraints between domains: 5 6

Local NCS retraints between domains: 7 8

Local NCS retraints between domains: 9 10

Local NCS retraints between domains: 11 12