Structure of DDB1 bound to Z7-engaged CDK12-cyclin K

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Design principles for cyclin K molecular glue degraders.

DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe

Cdc2-related kinase,arginine/serine-rich,CrkRS,Cell division cycle 2-related protein kinase 7,CDC2-related protein kinase 7,Cell division protein kinase 12,hCDK12

GGGRMSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDL
LFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFN
IRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQES
ITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTY
LDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGG
NGNSGEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHET
SFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQT
VAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEG
NFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGET
STPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDI
SRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

GGGRRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVN
MKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLN
NSGQIKLADFGLARLYNSEESRPY(TPO)NKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQ
LELISRLCGSPCPAVWPDVIRLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVE
LSKMAPPDLPHWQDCHELWSKKRRRQRQ

GGGRRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVN
MKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLN
NSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELI
SRLCGSPCPAVWPDVIRLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKM
APPDLPHWQDCHELWSKKRRRQRQ

GGGRMKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGII
YFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQ
VEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWE
QFVQDVPVDVLEDICHQILDLYSQGKQQMPH

1.50 M ammonium sulphate, 0.07 M HEPES, pH 7.0

Marie Sklodowska-Curie Actions, FragNET ITN

chain 'A' and (resid 1 through 307 )
chain 'A' and (resid 308 through 716 )
chain 'A' and (resid 717 through 828 )
chain 'A' and (resid 829 through 1140 )
chain 'B' and (resid 715 through 794 )
chain 'B' and (resid 795 through 832 )
chain 'B' and (resid 833 through 880 )
chain 'B' and (resid 881 through 901 )
chain 'B' and (resid 902 through 1020 )
chain 'B' and (resid 1021 through 1043 )
chain 'C' and (resid 20 through 42 )
chain 'C' and (resid 43 through 63 )
chain 'C' and (resid 64 through 151 )
chain 'C' and (resid 152 through 217 )
chain 'C' and (resid 218 through 243 )
chain 'C' and (resid 244 through 267 )
chain 'D' and (resid 1 through 365 )
chain 'D' and (resid 366 through 819 )
chain 'D' and (resid 820 through 1140 )
chain 'E' and (resid 715 through 768 )
chain 'E' and (resid 769 through 794 )
chain 'E' and (resid 795 through 832 )
chain 'E' and (resid 833 through 875 )
chain 'E' and (resid 876 through 896 )
chain 'E' and (resid 897 through 931 )
chain 'E' and (resid 932 through 980 )
chain 'E' and (resid 981 through 1026 )
chain 'E' and (resid 1027 through 1043 )
chain 'F' and (resid 20 through 42 )
chain 'F' and (resid 43 through 106 )
chain 'F' and (resid 107 through 135 )
chain 'F' and (resid 136 through 150 )
chain 'F' and (resid 151 through 218 )
chain 'F' and (resid 219 through 233 )
chain 'F' and (resid 234 through 267 )
chain 'G' and (resid 1 through 307 )
chain 'G' and (resid 308 through 795 )
chain 'G' and (resid 796 through 856 )
chain 'G' and (resid 857 through 1140 )
chain 'H' and (resid 715 through 768 )
chain 'H' and (resid 769 through 794 )
chain 'H' and (resid 795 through 832 )
chain 'H' and (resid 833 through 872 )
chain 'H' and (resid 873 through 890 )
chain 'H' and (resid 891 through 952 )
chain 'H' and (resid 953 through 1021 )
chain 'H' and (resid 1022 through 1043 )
chain 'I' and (resid 20 through 42 )
chain 'I' and (resid 43 through 63 )
chain 'I' and (resid 64 through 150 )
chain 'I' and (resid 151 through 217 )
chain 'I' and (resid 218 through 243 )
chain 'I' and (resid 244 through 267 )
kinase, cyclin, ubiquitin, degrader, LIGASE
(chain "A" and (resid 1 through 393 or resid 709 through 1140))
(chain "D" and (resid 1 through 393 or resid 709 through 1140))
(chain "G" and (resid 1 through 393 or resid 709 through 1140))

MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFIL
TAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLE
ELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYH
NGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNG
VVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIG

KLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEV
HNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKG
AVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARD
FNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSV
LFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEV
VANLQYDDGSGMKREATADDLIKVVEELTRIH

RRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKE
IVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSG
QIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRL
CGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPP
DLPHWQDCHELWSKKRRRQR

MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHR
FYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHP
YQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQ
DVPVDVLEDICHQILDLYSQGKQQMPH