Cryo-EM structure of the bacterial replication origin opening basal unwinding system

The bacterial replication origin BUS promotes nucleobase capture.

Chromosomal replication initiator protein DnaA

DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3')

MENILDLWNQALAQIEKKLSKPSFETWMKSTKAHSLQGDTLTITAPNEFARDWLESRYLHLIADTIYELTGEELSIKFVI
PQNQDVEDFMPKPQVKKAVKEDTSDFPQNMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHL
MHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEES
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQIDSNIRELEGALI
RVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKEIQRVVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDS
SLPKIGEEFGGRDHTTVIHAHEKISKLLADDEQLQQHVKEIKEQLK

(DA)(DC)(DT)(DT)(DA)(DT)(DC)(DC)(DA)(DC)(DA)(DA)(DA)(DT)(DC)(DC)(DA)(DC)(DA)(DG)
(DG)(DC)(DC)(DC)

(DT)(DA)(DG)(DT)(DA)(DG)(DA)(DA)(DG)(DT)(DA)(DA)(DT)(DA)(DG)(DT)(DA)(DG)(DG)(DG)
(DC)(DC)(DT)(DG)(DT)(DG)(DG)(DA)(DT)(DT)(DT)(DG)(DT)(DG)(DG)(DA)(DT)(DA)(DA)(DG)
(DT)

Cryo-EM structure of the DnaA domain III lattice of the BUS complex

Cryo-EM map of the region around the dsDNA of the BUS complex

Cryo-EM structure of the BUS complex - domain IV lattice

DnaA, cryo-EM, BUS complex, replication initiation, REPLICATION