Dysgonamonadaceae bacterium CRISPR ancillary nuclease 2

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Reverse transcriptases prime DNA synthesis.

MGSSHHHHHHSSGLVPRGSHMSHVQITLVGGQAAPVYNGITYYNPDKVILVCSKQTQNEAMRIKAEFPDIAEIKVMDPVN
IAEIVSETRALADSMPDDEIYVNISGGTKSWAFYFSRIFSERSNTKIFYIDQNNTIWNFTDQTHSQANFDLNLDVQFRLY
GNSLKEYKLVSDFADDDLTIIPKIYKIRSFDKRNFGKLMNLYSENSENVFFDLDNGSYLRWDNEQQLFEINIRNRDGQSK
HEILKSTHIRRLLRNYTWLELEIARVLSGWKFAKEVRLNGIFRDKHENAKNEIDCIVNLGNKILFVECKSHITNITDIDK
FKNAVKVYGGSGCKALFTTIDPIRNDALEKCRDSNIIPFCIEKNGGINNYKSNLFEILEKEILNINP

0.1M Imidazole; MES monohydrate (acid)
0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide
20% v/v Glycerol; 10% w/v PEG 4000
208.3uM cA4

Biotechnology and Biological Sciences Research Council (BBSRC)

nuclease, homodimer, can2, CRISPR ancillary nuclease, cyclic tetra-adenylate, DNA BINDING PROTEIN

MSHVQITLVGGQAAPVYNGITYYNPDKVILVCSKQTQNEAMRIKAEFPDIAEIKVMDPVNIAEIVSETRALADSMPDDEI
YVNISGGTKSWAFYFSRIFSERSNTKIFYIDQNNTIWNFTDQTHSQANFDLNLDVQFRLYGNSLKEYKLVSDFADDDLTI
IPKIYKIRSFDKRNFGKLMNLYSENSENVFFDLDNGSYLRWDNEQQLFEINIRNRDGQSKHEILKSTHIRRLLRNYTWLE
LEIARVLSGWKFAKEVRLNGIFRDKHENAKNEIDCIVNLGNKILFVECKSHITNITDIDKFKNAVKVYGGSGCKALFTTI
DPIRNDALEKCRDSNIIPFCIEKNGGINNYKSNLFEILEKEILNINP