Legionella effector Lem3 mutant D190A in complex with Mg2+

Dephosphocholination by Legionella effector Lem3 functions through remodelling of the switch II region of Rab1b.

GHDKIITGKKIIFSQSVAKDQTKNLSSFLSERFYSVNQSHNHSIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNG
ARVAITDGLGGGNGDQEEDDEIYRVSHSSCENFLNCDQNIDTTLSLITQPKASDKKQTAPKTLQHTEAS(MSE)AAFIYQ
NHPGKGYIGEFANIGAGLIIILDKRFKIKH(MSE)VSACHIYRGFGTWTPPSLQALATTANKDALLVRQTLKLAEGDIII
S(MSE)TDGVWGELKTSLIAQTNDRRDIGVDKEYFKTLFDELTDAPYPSSFDIARIITQRA(MSE)SRSLERRKTLIKLI
NEIEQQHFHEKSVKTINEVLEYFIKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLE(MSE)V(MSE)HDLKSRTVGD
CSTINVTRIPYHLDELIRGFINYPEKHQILAPLFKARVKSEADLEEAFHRLSLE(MSE)VQPEIECPISETHFERAFKKE
TLDKTQAVLTHYFRIS

GHDKIITGKKIIFSQSVAKDQTKNLSSFLSERFYSVNQSHNHSIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNG
ARVAITDGLGGGNGDQEEDDEIYRVSHSSCENFLNCDQNIDTTLSLITQPKASDKKQTAPKTLQHTEASMAAFIYQNHPG
KGYIGEFANIGAGLIIILDKRFKIKHMVSACHIYRGFGTWTPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGVWG
ELKTSLIAQTNDRRDIGVDKEYFKTLFDELTDAPYPSSFDIARIITQRAMSRSLERRKTLIKLINEIEQQHFHEKSVKTI
NEVLEYFIKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTVGDCSTINVTRIPYHLDELIRGFINYP
EKHQILAPLFKARVKSEADLEEAFHRLSLEMVQPEIECPISETHFERAFKKETLDKTQAVLTHYFRIS

Lem3 determined by SAD (thiomersal derivate)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

Legionella effector Lem3 displaces the switch II region of Rab1b for dephosphocholination, METAL BINDING PROTEIN

DKIITGKKIIFSQSVAKDQTKNLSSFLSERFYSVNQSHNHSIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNGAR
VAITDGLGGGNGDQEEDDEIYRVSHSSCENFLNCDQNIDTTLSLITQPKASDKKQTAPKTLQHTEASMAAFIYQNHPGKG
YIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTWTPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGVWGEL
KTSLIAQTNDRRDIGVDKEYFKTLFDELTDAPYPSSFDIARIITQRAMSRSLERRKTLIKLINEIEQQHFHEKSVKTINE
VLEYFIKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTVGDCSTINVTRIPYHLDELIRGFINYPEK
HQILAPLFKARVKSEADLEEAFHRLSLEMVQPEIECPISETHFERAFKKETLDKTQAVLTHYFRIS