Crystal structure of a chimeric LOV-Histidine kinase SB2F1-I66R mutant (asymmetrical variant, trigonal form with long c axis)

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

HIS-TAG: 1-MGSSHHHHHHSSGLVPRGSH-20;
BELONGS TO Q88JB0: 21 (OR 1 PDB-FILE)-MINA...YYIGIQRDVT-140 (OR 120);
BELONGS TO P23222:141 (OR 121 PDB-FILE)-EHQQTQ...AADEN-388 (OR 368);
UNP:Q88JB0 (UNP-NUMBERING) 4-MINA...QRDVT-123;
UNP:P23222 (UNP-NUMBERING) 258 TO C-TERM;
ENGINEERED MUTATION: ILE 66 ARG.

Putative Sensory box protein,Sensor protein FixL

MGSSHHHHHHSSGLVPRGSHMINAKLLQLMVEHSNDGIVVAEQEGNESILIYVNPAFERLTGYCADDILYQDCRFLQGED
HDQPGRAIIREAIREGRPCCQVLRNYRKDGSLFWNELSITPVHNEADQLTYYIGIQRDVTEHQQTQARLQELQSELVHVS
RLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVARGESEKRVE
SLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNLFRNALEAMAQSQRRELVVTNTPAADDMIEVE
VSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAADEN

Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)

9% PEG8K, 0.1M Na-Citrate, 0.2M NaCl, 1mM ATP, 2mM MgCl2

German Federal Ministry for Education and Research

chain 'A' and (resid 0 through 176 )

chain 'A' and (resid 177 through 362 )

chain 'A' and (resid 401 through 401 )

chain 'A' and (resid 402 through 402 )

chain 'B' and (resid -2 through 119 )

chain 'B' and (resid 120 through 174 )

chain 'B' and (resid 175 through 362 )

chain 'B' and (resid 401 through 401 )

chain 'B' and (resid 402 through 402 )

LOV domain, PAS domain, Photocycle, Dimerization, Signaling blue light photoreceptor, sensory histidine kinase, chimeric, De Novo Protein, I66R mutant, SIGNALING PROTEIN

(chain "B" and (resid 0 through 362 or resid 401 through 402))

MINAKLLQLMVEHSNDGIVVAEQEGNESILIYVNPAFERLTGYCADDILYQDCRFLQGEDHDQPGIAIIREAIREGRPCC
QVLRNYRKDGSLFWNELSITPVHNEADQLTYYIGIQRDVT

EHQQTQARLQELQSELVHVSRLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAG
QIIRRLRDFVARGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNLFRNALEAMAQS
QRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHGGRMWAESNASGGATFRF
TLPAADEN