Crystal structure of evolved photoenzyme EnT1.3

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

A designed photoenzyme for enantioselective [2+2] cycloadditions.

MEIPVIEPLFTKVTEDIPGAAGPVFDKNGDFYIVAPYVEVNGKPAGEILRIDLKTGKKTVICKPEVNGYGGIPAGCQCDR
DANQLFVADARLGLLVVQTDGTFEEIAKKDSEGRRMQGCAYCAFDYEGNLWITAPAGEVAPADFTISLDEKFGSIYCFTT
DGQMIQVDTAFQ(PBF)PAGIAVRHMNDGRPYQLIVAEQRTKKLWSYDIKGPAKIENKKVWGHIPGTHEGGASGMDFDED
NNLLVANWGSSHIEVFGPDGGQPKMRIRCPFEKPSALHFKPQTKTIFVTEHENNAVWKFEWQRNGKKQYCETLKFGIFGS
LEHHHHHH

MEIPVIEPLFTKVTEDIPGAAGPVFDKNGDFYIVAPYVEVNGKPAGEILRIDLKTGKKTVICKPEVNGYGGIPAGCQCDR
DANQLFVADARLGLLVVQTDGTFEEIAKKDSEGRRMQGCAYCAFDYEGNLWITAPAGEVAPADFTISLDEKFGSIYCFTT
DGQMIQVDTAFQFPAGIAVRHMNDGRPYQLIVAEQRTKKLWSYDIKGPAKIENKKVWGHIPGTHEGGASGMDFDEDNNLL
VANWGSSHIEVFGPDGGQPKMRIRCPFEKPSALHFKPQTKTIFVTEHENNAVWKFEWQRNGKKQYCETLKFGIFGSLEHH
HHHH

0.2 M Ammonium chloride, 0.1 M HEPES, pH = 7.0, 20% w/v PEG 6000

Biotechnology and Biological Sciences Research Council (BBSRC)

Engineering and Physical Sciences Research Council

Designed photoenzyme, [2+2]-cyclase, genetic code expansion, Engineered enzyme, BIOSYNTHETIC PROTEIN

MEIPVIEPLFTKVTEDIPGAEGPVFDKNGDFYIVAPEVEVNGKPAGEILRIDLKTGKKTVICKPEVNGYGGIPAGCQCDR
DANQLFVADMRLGLLVVQTDGTFEEIAKKDSEGRRMQGCNDCAFDYEGNLWITAPAGEVAPADYTRSMQEKFGSIYCFTT
DGQMIQVDTAFQFPNGIAVRHMNDGRPYQLIVAETPTKKLWSYDIKGPAKIENKKVWGHIPGTHEGGADGMDFDEDNNLL
VANWGSSHIEVFGPDGGQPKMRIRCPFEKPSNLHFKPQTKTIFVTEHENNAVWKFEWQRNGKKQYCETLKFGIF