Tail tip of siphophage T5 : central fibre protein pb4

Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation.

Pure T5 tails obtained by infecting E. coli F strain with the amber mutant phage T5D20am30d, incubated with E. coli receptor FhuA reconstituted into nanodisc

3 uL of T5 tails sample (with or without FhuA-ND) were deposited on a freshly glow discharged EM grid and plunge-frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100 percent humidity, 20 Celsius degrees, 5 s blotting time, blot force 0)

MISNNAPAKMVLNSVLTGYTLAYIQHSIYSDYDVIGRSFWLKEGSNVTRRDFTGIDTFSVTINNLKPTTTYEVQGAFYDS
IIDSELLNAQIGINLSDKQTFKMKSAPRITGARCESEPVDVGVGAPIVYIDTTGEADYCTIELKDNSNANNPWVKYYVGA
LMPTIMFGGVPIGSYKVRISGQISLPDGVTIDSSGYYEYPNVFEVRYNFVPPAAPINIVFKAARIADGKERYDLRVQWDW
NRGAGANVREFVLSYIDSAEFVRTGWTKAQKINVGAAQSATIISFPWKVEHKFKVSSIAWGPDAQDVTDSAVQTFILNES
TPLDNSFVNETGIEVNYAYIKGKIKDGSTWKQTFLIDAATGAINIGLLDAEGKAPISFDPVKKIVNVDGSVITKTINAAN
FVMTNLTGQDNPAIYTQGKTWGDTKSGIWMGMDNVTAKPKLDIGNATQYIRYDGNILRISSEVVIGTPNGDIDIQTGIQG
KQTVFIYIIGTSLPAKPTSPAYPPSGWSKTPPNRTSNTQNIYCSTGTLDPVTNQLVSGTSWSDVVQWSGTEGVDGRPGAT
GQRGPGMYSLAIANLTAWNDSQANSFFTSNFGSGPVKYDVLTEYKSGAPGTAFTRQWNGSAWTSPAMVLHGDMIVNGTVT
ASKIVANNAFLSQIGVNIIYDRAAALSSNPEGSYKMKIDLQNGYIHIR

Bacteriophage, Siphophage, T5, baseplate, VIRAL PROTEIN, VIRUS LIKE PARTICLE