X-31 Hemagglutinin Precursor HA0 at pH 4.8

alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Reversible structural changes in the influenza hemagglutinin precursor at membrane fusion pH.

Uncleaved precursor form of the X-31 influenza hemagglutinin with the T4 fibritin foldon attached to the C-terminus.

Influenza A virus (A/Aichi/2/1968(H3N2))

Images were subject to whole-frame motion correction and dose weighting.

15696 images on session 1,
18281 images on session 2.

646k from session 1,
589k from session 2.

The sample protein tended to aggregate and bind to the carbon film. 0.1% b-octyl glucoside was added.

T4 fibritin foldon attached to the C-terminal of HA0 as a trimerization domain. C-terminal His8-tag for protein purification.,T4 fibritin foldon attached to the C-terminal of HA0 as a trimerization domain. C-terminal His8-tag for protein purification.,T4 fibritin foldon attached to the C-terminal of HA0 as a trimerization domain. C-terminal His8-tag for protein purification.,T4 fibritin foldon attached to the C-terminal of HA0 as a trimerization domain. C-terminal His8-tag for protein purification.

Collar protein,Whisker antigen control protein

QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQ
NETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGST
YPVLNVTMPNNDNFDKLYIWGIHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPG
DVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGM
RNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFS
EVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEEMGNGCFKIYHKCDNACIES
IRNGTYDHDVYRDEALNNRFQIKGGGRENLYFQGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHHHH

virus envelope, receptor binding, membrane fusion, VIRAL PROTEIN

QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQ
NETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGST
YPVLNVTMPNNDNFDKLYIWGIHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPG
DVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGM
RNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFS
EVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEEMGNGCFKIYHKCDNACIES
IRNGTYDHDVYRDEALNNRFQIKG