Cryo-EM structure of compact CA16 empty particle in complex with a neutralizing antibody 8C4

Molecular mechanism of antibody neutralization of coxsackievirus A16.

HSTQETAIGNFFSRAGLVSIITMPTMGTQNTDGYANWDIDLMGYAQLRRKCELFTYMRFDAEFTFVVAKPNGELVPQLLQ
YMYVPPGAPKPTSRDSFAWQTATNPSVFVKMTDPPAQVSVPFMSPASAYQWFYDGYPTFGEHLQANDLDYGQCPNNMMGT
FSIRTVGTKKSPHSITLRVYMRIKHVRAWIPRPLRNQPYLFKTNPNYKGNDIKCTSTSRDKITTL

DIQMTQSSSYLSVSLGGRVTITCKASDHINNWLAWYQQKPGNAPRLLISGATSLETGVPSRFSGSGSGKDYTLSITSLQT
EDVATYYCQQYWNSPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVL
NSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

ENSNSASEGSTINYTTINYYKDAYAASAGRQDMSQDPKRFTDPVMDVIHEMAPPLKSPSAEACGYSDRVAQLTIGNSTIT
TQEAANIVIAYGEWPEYCPDTDATAVDKPTRPDVSVNRFFTLDTKSWAKDSKGWYWKFPDVLTEVGVFGQNAQFHYLYRS
GFCVHVQCNASKFHQGALLVAVLPEYVLGTIAGGTGNENSHPPYATTQPGQVGAVLTHPYVLDAGIPLSQLTVCPHQWIN
LRTNNCATIIVPYMNTVPFDSALNHCNFGLLVIPVVPLDFNAGATSEIPITVTIAPMCAEFAGLRQAVKQ

QVQLQQSGPELVKPGASVKISCKASGYAFSTSWMNWVIQRPGQGLEWIGRIYPGDGDTNYNGKFKGKATLTADKSSSTAY
MQLSSLTSVDSAVYFCARRDYGYFDYWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNS
GSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKL

GIPTELKPGTNQFLTTDDGVSAPILPGFHPTPPIHIPGEVRNLLEICRVETILEVNNLKTNETTPMQRLCFPVSVQSKTG
ELCAAFRADPGRDGPWQSTILGQLCRYYTQWSGSLEVTFMFAGSFMATGKMLIAYTPPGGSVPADRITAMLGTHVIWDFG
LQSSVTLVVPWISNTHYRAHARAGYFDYYTTGIITIWYQTNYVVPIGAPTTAYIVALAAAQDNFTMKLCKDTEDIEQTAN
IQ

icosahedral asymmetric unit, std point frame

coxsackievirus A16, antibody 8C4, cryo-EM, STRUCTURAL PROTEIN

HSTQETAIGNFFSRAGLVSIITMPTMGTQNTDGYVNWDIDLMGYAQLRRKCELFTYMRFDAEFTFVVAKPNGELVPQLLQ
YMYVPPGAPKPTSRDSFAWQTATNPSVFVKMTDPPAQVSVPFMSPASAYQWFYDGYPTFGEHLQANDLDYGQCPNNMMGT
FSIRTVGTKKSPHSITLRVYMRIKHVRAWIPRPLRNQPYLFKTNPNYKGNDIKCTSTSRDKITTL