Crystal structure of CYP109B4 from Bacillus Sonorensis

Rationally Controlling Selective Steroid Hydroxylation via Scaffold Sampling of a P450 Family

GMNSAKQQNPIQKALLNGKNRQDPYDPFPWYEKMRKESPVYYDEDSKVWSVFLYDDVKRVISDKDFFSNQFPQLESGNTF
AKTMVSMDPPKHTRIRSIVSKAFTPRIMKEWEPRIRVLTDELLGKARGRDEIDLVQDFSYPLPVMVISELLGVPSEHKEK
FKEWSDLLVSLPKSAYEEDVMEWRTIRNKGEEDLSAFFENVIEEKRRNLGDDIISLLIQAEEDGDRLSPDELVPFCNLLL
LAGNETTTNLISNMVYSILEKPGTFDELANQPDLIPQAVEEAVRFRAPAPMIVRFVQQDTAIRGVNLKKGEGVIAFLASA
NRDEAAFERAHEFDIHRHPNRHIGFGHGIHFCLGAPLARLETKIALEALLKQYSAMETISTEPMANSSMYGLKHFRLHVK
EALLSS

20% PEG 4000, 0.1M Na-Citrate pH 5.6, 20% iso-Propanol

substrate binding, complex, OXIDOREDUCTASE

MNSAKQQNPIQKALLNGKNRQDPYDPFPWYEKMRKESPVCYDEDSKVWNVFLYDDVKRVISDKDFFSNQFPQLESGNTFA
KTMVSMDPPKHTRIRSIVSKAFTPRIMKEWEPRIRVLTDELLGKARGRDEIDLVQDFSYPLPVMVISELLGVPSEHKEKF
KEWSDLLVSLPKSAYEEDVMEWRTIRNKGEEDLSAFFENVIEEKRRNLGDDIISLLIQAEEDGDRLSPDELVPFCNLLLL
AGNETTTNLISNMVYSILEKPGTLDELANQPDLIPQAVEEAVRFRAPAPMIVRFVQQDTAIRGVNLKKGEGVIAFLASAN
RDEAAFERAHEFDIHRHPNRHIGFGHGIHFCLGAPLARLETKIALEALLKQYSAMETISTEPMANSSMYGLKHFRLHVKE
ALLSS