Solution structures of ASH1L PHD domain in complex with H3K4me2 peptide
Structural insight into ASH1L PHD finger recognizing methylated histone H3K4 and promoting cell growth in prostate cancer.
Histone-lysine N-methyltransferase ASH1L
ASH1-like protein,huASH1,Absent small and homeotic disks protein 1 homolog,Lysine N-methyltransferase 2H
National Natural Science Foundation of China (NSFC)
3D 13C-edited 13C/15N-Filtered NOESY aliphatic
3D 13C-edited 13C/15N-Filtered NOESY aromatic
DGSA-distance geometry simulated annealing
50 mM sodium phosphate, 100 mM sodium chloride, 100 uM ZINC ION, 2.0 mM [U-100% 2H] DTT, 90% H2O/10% D2O
50 mM sodium phosphate, 100 mM sodium chloride, 100 uM ZINC ION, 2.0 mM [U-100% 2H] DTT, 100% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
ASH1L, PHD domain, H3K4me2, prostate cancer, STRUCTURAL PROTEIN