E.coli phosphoribosylpyrophosphate (PRPP) synthetase type B filament bound with Pi

Filamentation modulates allosteric regulation of PRPS.

E.coli PRPP syhthetase type B filament structure complex with Pi

Escherichia coli str. K-12 substr. MG1655

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

The purifide monomer or oligomer protein was incubated with Pi to form this type B filament.

blot for 3.5 seconds with blot force
of -1 before plunge-freezing

RPPK,5-phospho-D-ribosyl alpha-1-diphosphate,Phosphoribosyl diphosphate synthase,Phosphoribosyl pyrophosphate synthase,P-Rib-PP synthase,PRPP synthase,PRPPase

MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEHHHHHH
H

Ministry of Science and Technology (MoST, China)

National Natural Science Foundation of China (NSFC)

Allosteric enzyme, Kinase, Transferase, Nucleotide biosynthesis, ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, BIOSYNTHETIC PROTEIN

MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH