T4 lysozyme mutant-S44C/C54T/N68C/A93C/C97A/T115C, pH10

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Creation of Cross-Linked Crystals With Intermolecular Disulfide Bonds Connecting Symmetry-Related Molecules Allows Retention of Tertiary Structure in Different Solvent Conditions.

MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKCELDKAIGRNTNGVITKDEAEKLFCQDVDAAVRGILR
NAKLKPVYDSLDCVRRAALINMVFQMGETGVAGFCNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDA
YKNL

A crystal was soaked into precipitant-free solutions (pH10)

Intermolecular disulfide symmetry cross-linked crystal, HYDROLASE

MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNCNGVITKDEAEKLFNQDVDAAVRGILR
NAKLKPVYDSLDAVRRCALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDA
YKNL