Crystal structure of apo TrmM from Mycoplasma capricolum

Structural and functional characterization of TrmM in m 6 A modification of bacterial tRNA.

MRGSHHHHHHGRSGDDDDKMKVLNDLLGYKNRKLYQDNKMFNFTLDSVLVARFCNLNSKKKKICDFGTNNAVIPLILSKY
TKAKIIGVEIQNKAVEIANENIKLNGLEDQIEIVHADIKEFSKLHNQEFDLVVCNPPFFKMDGNPKLKEISLEVANARHE
ILITLEDIIKSASRCLKNKGNFTIVHRSERLSEIINLFYKYNIYPKRLRLIQSKKTDNAKMILLDGIYQGNEGMEILPTL
ITHNDDETYTDELLKYFHD

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

2.0 M Sodium chloride
10% w/v Polyethylene glycol 6,000

National Research Foundation (NRF, Korea)

t6A, m6A, methyltransferase, TRANSFERASE

MKVLNDLLGYKNRKLYQDNKMFNFTLDSVLVARFCNLNSKKKKICDFGTNNAVIPLILSKYTKAKIIGVEIQNKAVEIAN
ENIKLNGLEDQIEIVHADIKEFSKLHNQEFDLVVCNPPFFKMDGNPKLKEISLEVANARHEILITLEDIIKSASRCLKNK
GNFTIVHRSERLSEIINLFYKYNIYPKRLRLIQSKKTDNAKMILLDGIYQGNEGMEILPTLITHNDDETYTDELLKYFHD