A nonheme iron- and alpha-ketoglutarate- dependent halogenase that catalyzes nucleotide substrates

Structural and Functional Insights into a Nonheme Iron- and alpha-Ketoglutarate-Dependent Halogenase That Catalyzes Chlorination of Nucleotide Substrates.

nonheme iron and alpha-ketoglutarate dependent halogenase

AGAGAGAGAGMDVPLMELSGRAPVVRLHDIEADMAAATDAIRSQLTGWGFMAAEVPGIGERVEAMMNEFAAACRATGPSL
SDYAYDVVPQLAVGGTHGFFPYNSEIPRLANGVPDPKEFIHVSGAMIGDQPPGAGDVLRAFPAFGTRAAEVFDIAFRLIS
LFGEVVRGMMPPGTPELDLSHDATNLRVIHYRDVGDREVLAHEHSGIQMLGLQLPPSDQGLQYVLHDGTWVEPVIAGTDV
VLCNIGRMLTSASDGRFRPSTHRVHTKPMPAGYERLSSVLFAYPQHKARQWKMVDGELMSLNATWGDFIDSRFQGLGKQS

Ammonium Sulfate, HEPES pH 7.5, PEG 400, di-Potassium hydrogen phosphate

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

MDVPLMELSGRAPVVRLHDIEADMAAATDAIRSQLTGWGFMAAEVPGIGERVEAMMNEFAAACRATGPSLSDYAYDVVPQ
LAVGGTHGFFPYNSEIPRLANGVPDPKEFIHVSGAMIGDQPPGAGDVLRAFPAFGTRAAEVFDIAFRLISLFGEVVRGMM
PPGTPELDLSHDATNLRVIHYRDVGDREVLAHEHSGIQMLGLQLPPSDQGLQYVLHDGTWVEPVIAGTDVVLCNIGRMLT
SASDGRFRPSTHRVHTKPMPAGYERLSSVLFAYPQHKARQWKMVDGELMSLNATWGDFIDSRFQGLGKQS