Functional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated the Engineering Enhancement of Sugar Donor Promiscuity

Plant steroid glycosyltransferase UGT74AN2

GSHMGTIEISSPSKTHILAFPFPEKGHINPMLHLCNRLASKGFRVTLITTISTYKDVKNKIKCKSKGGLINLESIPDGTD
KNLGMNGYFNQFKNSVTESVAGIIEEYKLGHDFPPPKVLIYDSTMPWMLDVAHGHGILGASLFTQPCCVSVVYYHMLQGT
IDFHREQSSSSKVLLLPCLPPLEDRDLPEFDYFKEDSGFVSNLLLNQFLNIDKIDYVLFNTFEMLESEIANWMSNKWKIL
TIGPTAPTAAAAAAANNYLFETNTEVCMKWLDEREPNSVIYVSFGSIASLTEQQMEEISQALFTTNFNFLWVVREEERTK
LPNCLNNNNNNNNNPSSESFTTAAGKLGLIINWCPQLDVLRHESVACFMTHCGWNSTLEAISSGVPMICVPQWVDQTTNA
KFIQDVWKIGVRVNNNNGENGGLVKKEEIERCIKEVCESEKGKELKRNAMKWKDLSKEAVSEGGSSDTNLEYFASTLLFY

MGTIEISSPSKTHILAFPFPEKGHINPMLHLCNRLASKGFRVTLITTISTYKDVKNKIKCKSKGGLINLESIPDGTDKNL
GMNGYFNQFKNSVTESVAGIIEEYKLGHDFPPPKVLIYDSTMPWMLDVAHGHGILGASLFTQPCCVSVVYYHMLQGTIDF
HREQSSSSKVLLLPCLPPLEDRDLPEFDYFKEDSGFVSNLLLNQFLNIDKIDYVLFNTFEMLESEIANWMSNKWKILTIG
PTAPTAAAAAAANNYLFETNTEVCMKWLDEREPNSVIYVSFGSIASLTEQQMEEISQALFTTNFNFLWVVREEERTKLPN
CLNNNNNNNNNPSSESFTTAAGKLGLIINWCPQLDVLRHESVACFMTHCGWNSTLEAISSGVPMICVPQWVDQTTNAKFI
QDVWKIGVRVNNNNGENGGLVKKEEIERCIKEVCESEKGKELKRNAMKWKDLSKEAVSEGGSSDTNLEYFASTLLFY