E. coli Ribonuclease HI in complex one Zn2+ (His124 N-epsilon binding)

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Pivotal role of a conserved histidine in Escherichia coli ribonuclease HI as proposed by X-ray crystallography.

MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQ
WIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV

rnhA, dasF, herA, rnh, sdrA, b0214, JW0204

0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v polyethylene glycol monomethyl ether 2,000

Japan Society for the Promotion of Science (JSPS)