Cryo-EM structure of human NTCP complexed with YN69202Fab in the presence of myristoylated preS1 peptide

Structure of the bile acid transporter and HBV receptor NTCP.

25 mM Hepes-NaOH, pH 7.5, 0.15 M NaCl, and 0.01% GDN

Complex of NTCP and Fab in the presence of myristoylated preS1 peptide

Fab heavy chain from antibody IgG clone number YN69202

Fab light chain from antibody IgG clone number YN69202

Cell growth-inhibiting gene 29 protein,Na(+)/bile acid cotransporter,Na(+)/taurocholate transport protein,Sodium/taurocholate cotransporting polypeptide,Solute carrier family 10 member 1

MEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLG
KVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYIYSRGIYDGDLKDKVPYKGIV
ISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPLLIATSSLMPFIGFLLGYVL
SALFCLNGRCRRTVSMETGCANVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMI
ENLYFQGDYKDDDDKHHHHHHHH

EVQLQQSGPELVKPGASVKMSCKASGYTFTSYIIHWVKQKPGQGLEWIGYINPYNDGTKYNEKFKGKGTLTSDKSSSTAY
MELSSLTSEDSAVYYCARSYYDGIPHYFDYWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTV
TWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPISTINPCPPCKECHKCP
APNLEGGPS

DIVMTQSPSSLAVSAGEKVTMSCKSSQSLFNSRTRRNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLT
ISSVQAEDLAVYYCKQSYYLLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSER
QNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Ministry of Education, Culture, Sports, Science and Technology (Japan)

Hepatitis B virus (HBV), host entry receptor, bile acid transporter, taurocholate, Na+-coupled symporter, TRANSPORT PROTEIN

MEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLG
KVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYIYSRGIYDGDLKDKVPYKGIV
ISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPLLIATSSLMPFIGFLLGYVL
SALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKM