Cryo-EM structure of E.coli retron-Ec86 (RT-msDNA-RNA) at 3.2 angstrom

Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism.

RNA-directed DNA polymerase from retron EC86

MKSAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTADFRYRIYTVEKKGPEKRMRTIYQPSRELKALQGWVL
RNILDKLSSSPFSIGFEKHQSILNNATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKICCYKNLLP
QGAPSSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLSAQSMKKVVKARDFLFSIIPSEGLVINSKKTCISGPRSQ
RKVTGLVISQEKVGIGREKYKEIRAKIHHIFCGKSSEIEHVRGWLSFILSVDSKSHRRLITYISKLEKKYGKNPLNKAKT

(DG)(DA)(DA)(DA)(DG)(DT)(DT)(DG)(DC)(DG)(DC)(DA)(DC)(DC)(DC)(DT)(DT)(DA)(DC)(DG)
(DT)(DC)(DA)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DG)(DG)(DG)(DT)(DT)(DT)(DC)(DC)(DT)
(DG)(DG)(DT)(DT)(DG)(DG)(DC)(DT)(DC)(DG)(DG)(DA)(DG)(DA)(DG)(DC)(DA)(DT)(DC)(DA)
(DG)(DG)(DC)(DG)(DA)(DT)(DG)(DC)(DT)(DC)(DT)(DC)(DC)(DG)(DT)(DT)(DC)(DC)(DA)(DA)
(DC)(DA)(DA)(DG)(DG)(DA)(DA)(DA)(DA)(DC)(DA)(DG)(DA)(DC)(DA)(DG)(DT)(DA)(DA)(DC)
(DT)(DC)(DA)(DG)(DA)

GAAAGTTGCGCACCCTTACGTCAGAAAAAACGGGTTTCCTGGTTGGCTCGGAGAGCATCAGGCGATGCTCTCCGTTCCAA
CAAGGAAAACAGACAGTAACTCAGA

UGCGCACCCUUAGCGAGAGGUUUAUCAUUAAGGUCAACCUCUGGAUGUUGUUUCGGCAUCCUGCAUUGAAUCUGAGUUAC
U

National Natural Science Foundation of China (NSFC)

Reverse transcriptase, RNA BINDING PROTEIN, TRANSFERASE-DNA-RNA complex