Crystal structure of heterohexameric Sa2YoeB-Sa2YefM complex bound to 26bp-DNA

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

The two paralogous copies of the YoeB-YefM toxin-antitoxin module in Staphylococcus aureus differ in DNA binding and recognition patterns.

HMSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVL
ILSAWSHYD

MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

(DT)(DT)(DG)(DA)(DC)(DG)(DT)(DA)(DC)(DT)(DC)(DA)(DA)(DG)(DT)(DG)(DC)(DG)(DT)(DA)
(DC)(DG)(DC)(DT)(DA)(DT)

(DA)(DT)(DA)(DG)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DA)(DC)(DT)(DT)(DG)(DA)(DG)(DT)(DA)
(DC)(DG)(DT)(DC)(DA)(DA)

Staphylococcus aureus (strain NCTC 8325 / PS 47)

0.02 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 7.0, 15% v/v 2-Propanol, 0.001 M Hexamine cobalt (III) chloride, 0.001 M Spermine

National Natural Science Foundation of China (NSFC)

Staphylococcus aureus subsp. aureus NCTC 8325

chain 'F' and (resid 1 through 9 )

chain 'F' and (resid 10 through 51 )

chain 'F' and (resid 52 through 88 )

chain 'B' and (resid 1 through 12 )

chain 'B' and (resid 13 through 33 )

chain 'B' and (resid 34 through 42 )

chain 'B' and (resid 43 through 57 )

chain 'B' and (resid 58 through 69 )

chain 'B' and (resid 70 through 76 )

chain 'B' and (resid 77 through 85 )

chain 'C' and (resid 1 through 5 )

chain 'C' and (resid 6 through 33 )

chain 'C' and (resid 34 through 42 )

chain 'C' and (resid 43 through 56 )

chain 'A' and (resid 1 through 12 )

chain 'A' and (resid 13 through 22 )

chain 'A' and (resid 23 through 42 )

chain 'A' and (resid 43 through 58 )

chain 'E' and (resid 0 through 37 )

chain 'E' and (resid 38 through 88 )

chain 'M' and (resid 1 through 22 )

chain 'M' and (resid 23 through 51 )

chain 'M' and (resid 52 through 88 )

chain 'L' and (resid 1 through 12 )

chain 'L' and (resid 13 through 42 )

chain 'L' and (resid 43 through 57 )

chain 'L' and (resid 58 through 69 )

chain 'L' and (resid 70 through 85 )

chain 'I' and (resid 1 through 37 )

chain 'I' and (resid 38 through 56 )

chain 'K' and (resid 1 through 23 )

chain 'K' and (resid 24 through 29 )

chain 'K' and (resid 30 through 42 )

chain 'K' and (resid 43 through 58 )

chain 'N' and (resid 1 through 37 )

chain 'N' and (resid 38 through 51 )

chain 'N' and (resid 52 through 56 )

chain 'N' and (resid 57 through 82 )

chain 'N' and (resid 83 through 88 )

chain 'D' and (resid 1 through 12 )

chain 'D' and (resid 13 through 29 )

chain 'D' and (resid 30 through 42 )

chain 'D' and (resid 43 through 57 )

chain 'D' and (resid 58 through 76 )

chain 'D' and (resid 77 through 84 )

chain 'J' and (resid 1 through 5 )

chain 'J' and (resid 6 through 22 )

chain 'J' and (resid 23 through 33 )

chain 'J' and (resid 34 through 42 )

chain 'J' and (resid 43 through 57 )

chain 'J' and (resid 58 through 68 )

chain 'J' and (resid 69 through 76 )

chain 'J' and (resid 77 through 85 )

chain 'G' and (resid 1 through 10 )

chain 'G' and (resid 11 through 26 )

chain 'H' and (resid 1 through 10 )

chain 'H' and (resid 11 through 25 )

chain 'P' and (resid 1 through 5 )

chain 'P' and (resid 6 through 10 )

chain 'P' and (resid 11 through 26 )

chain 'O' and (resid 1 through 10 )

chain 'O' and (resid 11 through 15 )

chain 'O' and (resid 16 through 20 )

chain 'O' and (resid 21 through 26 )

Toxin-antitoxin-DNA ternary complex, DNA BINDING PROTEIN, DNA BINDING PROTEIN-ANTITOXIN-DNA complex

MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD